番茄黄化曲叶病毒病抗性材料的筛选鉴定  

ScreeningandIdentificationofTomatoAnti-virus Variety Against Tomato Yellow Leaf Curl Virus

作　　者：田兆丰[1] 卢向阳[1] 厚凌宇[1] 罗晨[1] 柴敏[2] 

机构地区：[1]北京市农林科学院植物保护环境保护研究所,北京100097 [2]北京市农林科学院蔬菜研究中心,北京100097

出　　处：《科技导报》2014年第12期31-35,共5页Science & Technology Review

基　　金：公益性行业(农业)科研专项(201003065);北京市自然科学基金项目(6122017);现代农业产业技术体系北京市创新团队建设专项(blvt-13)

摘　　要：为评估新培育番茄栽培品种对番茄黄化曲叶病毒（tomato yellow leaf curl virus，TYLCV）的抗性水平，更全面地了解这些品种的抗病性差异，以金棚1号和金曼为感病对照，采用带毒烟粉虱自然传毒方式，对各品种的发病时间、发病率及病情指数等参数进行比较。结合PCR及ELISA对TYLCV的检测结果，综合分析了18个番茄品种对番茄黄化曲叶病毒的抗病性。结果表明，不同品种对TYLCV的抗性差异较大。金棚1号和金曼两份材料发病率都达到100％，病情指数在65．2以上，属于典型的感病品种：秋光15—6、PC-88和秋光69号3份材料发病率和病情指数均为0，属于抗性较高的品种；其他13份材料的发病率和病情指数分别在5．6％～45．2％和2．6—18．0之间，分别表现了不同程度的抗、耐病水平。To assess the resistance level of the new anti-virus tomato variety against tomato yellow leaf curl virus, the time of the virus affection, the disease incidence and the disease index of 181 tomato varieties are recorded in the field by the natural transmission with Jinpeng l and Jinman as the susceptible control. The resistance level is evaluated by PCR and ELISA. It is shown that the resistance varies significantly among the 18 tomato varieties. Two cultivars of Jinpengland Jinman are very susceptible, the disease incidences both reach 100% and the disease indices are both above 65.2. Three cuhivars of Qiuguang 15-6, PC-88, and Qiuguang 69 show a high level resistance, the disease incidences and the disease indices are all 0. The rest 13 cuhivars show different degrees of resistance levels, the disease incidences and the disease indices are between 5.6% and 45.2%, and 2.6 and 18.0, respectively.

关 键 词：番茄品种 番茄黄化曲叶病毒 抗性鉴定 

分 类 号：S436.412.1[农业科学—农业昆虫与害虫防治]