乙型肝炎孕妇胎盘组织中TNF-α的表达研究  

Study on expression of TNF- α in placenta of pregnant women infected with hepatitis B virus

作　　者：陈萱[1] 刘艳庚 吕楠[1] 赵姝妤[1] 

机构地区：[1]哈尔滨医科大学附属第二医院产科,黑龙江哈尔滨150086 [2]中航工业哈尔滨242医院产科,黑龙江哈尔滨150066

出　　处：《中国优生与遗传杂志》2014年第3期74-76,120,F0004,共5页Chinese Journal of Birth Health & Heredity

基　　金：黑龙江省卫生厅课题2004126

摘　　要：目的通过检测受到及未受到乙型肝炎病毒感染的孕妇胎盘组织中肿瘤坏死因子(TNF-α),并对二者加以比较探讨受HBV感染的孕妇胎盘组织中细胞介导的免疫应答反应情况。方法将实验对象分为三组:实验组1为39例血清学乙型肝炎表面抗原(HBsAg)阳性且乙型肝炎e抗原(HBeAg)阴性的孕妇的胎盘组织,试验组2为21例血清HBsAg阳性且HBeAg阳性的孕妇的胎盘组织,对照组为30例正常健康孕妇胎盘组织。采用SP法对各组的胎盘组织进行免疫组化染色检测TNF-α,观察各组TNF-α的定位和表达量。结果各组均可见TNF-α的表达。其定位各组均一致,阳性细胞主要表达在合体滋养细胞、血管内皮细胞,其次,还可见于蜕膜细胞、平滑肌细胞、绒毛间质细胞等。实验组2 TNF-α表达量与实验组1、对照组相比显著增高(P<0.05),实验组1 TNF-α表达量与对照组相比显著增高(P<0.05)。结论当孕妇受到HBV感染时,胎盘中T淋巴细胞介导的TNF-α表达增强,且胎盘中TNF-α表达高低在一定程度上反映了血清中乙型肝炎病毒感染的强弱。Objective： To investigate the cellular immune response in placenta of pregnant women infected with hepatitis B virus and to compare it with that of the controls by detecting TNF- α in placentas. Methods： Immunohistochemical techniques were used to show the location and quantity of TNF- α in placental tissue sections in the following three groups： HBV group 1 of placentas from 39 women whose pregnancies were complicated by infection of HBsAg but not HBeAg in their blood serum. HBV group 2 of placentas from 21 patients whose pregnancies were complicated by infection of HBsAg and HBeAg in their blood serum. Placentas from HBsAg negative pregnant women is control group. Results： Expression of TNF- α could be observed in both study groups and control groups, there was no difference in accord of the location of TNF- α. TNF- α positive cells were mainly located in syncytiotrophoblast,villous capillary endothelial cells,decisual cells and smooth muscle cells,villous mesenchymal cells etc. However,the quantity of TNF- α in study groups 2 was significantly higher than in study groups 1 and control groups（ P 0. 05）. The quantity of TNF- α in study groups 1 was significantly higher than control groups（ P 0. 05）. Conclusion： These findings demonstrate an inflammation- mediated cellular immune response of TNF- α in the placentas from women whose pregnancies are complicated with HBV infection in their placentas. The level or expression of TNF- α in the placentas from women whose pregnancies are complicated with HBV reflected the grade of the blood serum infection HBV to some degree.

关 键 词：乙型肝炎病毒 肿瘤坏死因子Α 胎盘 

分 类 号：R512.62[医药卫生—内科学]