孕妇血浆循环DNA的检测与动态分析  

Study of the quantitative change of circulating DNA in maternal plasma

作　　者：冉贵萍[1] 吴卫华[1] 谢艳平[1] 张圣芳[2] 方文[2] 

机构地区：[1]上海交通大学医学院附属第九人民医院奉城分院,上海201411 [2]贵阳医学院附属医院,贵阳550004

出　　处：《中国优生与遗传杂志》2014年第4期6-7,40,共3页Chinese Journal of Birth Health & Heredity

摘　　要：目的本文旨在探索使用荧光定量PCR方法对孕妇全血和血浆中DNA含量进行动态分析,从而为利用孕母血浆循环DNA进行无创产前诊断奠定基础。方法提取60例早、中、晚孕期孕妇全血基因组DNA和血浆循环DNA,应用实时荧光定量聚合酶链式反应(FQ-PCR)技术测定不同孕期的孕妇全血和血浆中保守基因(β-actin)的含量,比较不同孕期孕妇全血和血浆DNA含量的变化。结果 (1)早、中、晚孕期孕妇全血DNA含量Ct平均值为18.44±0.56828、18.35±0.93394、18.15±0.57410,3组比较没有显著性差异(P>0.05);(2)孕妇早、中、晚期孕组血浆DNA含量Ct平均值25.59±1.17673、25.50±1.59892、24.35±2.24301,早、中期孕组血浆DNA没有显著性差异(P>0.05),早、晚期和中、晚期孕组血浆DNA在两者之间有显著性差异(P<0.05);(3)排除全血DNA干扰,早、中、晚孕期孕妇血浆中DNA相对定量值为0.007401、0.007401、0.013602。结论用实时荧光定量PCR的方法可以检测到孕妇血浆中循环DNA的含量变化,随孕周的增加而逐渐增加,可以利用其进行无创产前诊断。Objective： To evaluate the quantitative change of circulating DNA in maternal plasma by real - time fluorescent quanti- tative polymerase chain reaction （ FQ - PCR）, so as to lay the foundation for non - invasive prenatal diagnosis. Methods： Whole blood DNA and plasma DNA was extracted from sixty eases of early, middle and late pregnancy women respectively. FQ - PCR was used to determine β - actin gene concentration in different stage of pregnancy, whole blood and plasma DNA levels were compared in three different pregnancy stage groups. Results ： （ 1 ） The mean Ct values of whole blood DNA in the early, middle and late pregnancy groups were 18.44 ± 0. 56828, 18. 35 ± O. 93394, 18. 15 ± O. 57410, there is no significant difference between the three groups （P 〉 0. 05）. （2） The mean Ct values of plasma DNA in the three groups were 25. 59 ± 1. 17673, 25.50 ± 1. 59892, 24. 35 ± 2. 24301, with significant difference between the early and late group （P 〈 0. 05 ） and with significant difference between the middle and late group （P 〈 O. 05 ）. （3） The relative quantitative values of circulating DNA in early, middle and late groups were 0. 007401, 0. 007401, O. 013602. Conclusion： The circulating DNA in maternal plasma could be detected by FQ -PCR and it increased with ges- tational aging, which may provide a theoretical basis for developing non - invasive prenatal diagnostic methods.

关 键 词：孕妇血浆 循环DNA β—actin 

分 类 号：R714.5[医药卫生—妇产科学]