银杏内酯B对缺氧缺血性脑损伤大鼠脑组织Foxg1 mRNA表达及内源性神经干细胞增殖的影响  

Effect of ginkgolide B on Foxg1 mRNA expression and endogenous neural stem cell proliferation of cerebral tissue of rats with hypoxic-ischemic brain damage

作　　者：王军[1] 张冬秀[1] 王宝珍[1] 朱登纳[1] 尚凤伟[1] 马洁琼[1] 陈静[1] 

机构地区：[1]郑州大学第三附属医院小儿脑瘫康复科,河南郑州450052

出　　处：《中国妇幼保健》2014年第14期2233-2237,共5页Maternal and Child Health Care of China

基　　金：河南省教育厅自然科学研究项目〔2009A320039〕

摘　　要：目的:探讨银杏内酯B(GB)对缺氧缺血性脑损伤(HIBD)新生大鼠脑组织Foxg1 mRNA的表达及内源性神经干细胞增殖、分化的影响。方法:清洁级新生7天SD大鼠随机分为假手术组、模型组及GB干预组,后两组采用Rice法建立HIBD模型,造模后3、7、14和28天处死,实时荧光定量PCR(RT-PCR)法检测脑组织Foxg1 mRNA的表达,免疫组化染色法检测海马齿状回BrdU+细胞的表达,免疫荧光双标法检测大脑皮层BrdU+/Nestin+、BrdU+/GFAP+和BrdU+/NSE+细胞的表达。结果:HIBD后各时间点脑组织Foxg1 mRNA、BrdU+细胞、BrdU+/Nestin+细胞的表达GB干预组>模型组>假手术组,差异有统计学意义(P<0.01);各时间点脑组织BrdU+/NSE+细胞的表达GB干预组>模型组>假手术组,差异有统计学意义(P<0.01);各时间点脑组织BrdU+/GFAP+细胞的表达GB干预组>模型组>假手术组,差异有统计学意义(P<0.01)。结论:GB通过上调HIBD后脑组织Foxg1 mRNA的表达,促进神经干细胞增殖及分化,减轻并修复脑损伤。Objective： To explore the effect of ginkgolide B （GB） on Foxgl mRNA expression and endogenous neural stem cell proliferation and differentiation of cerebral tissue of rats with hypoxic -ischemic brain damage （HIBD） . Methods： All the seven -day old clean Sprague Dawley rats were randomly divided into three groups： sham -operation group, model group and GB intervention group. HIBD model was established by Rice method in model group and GB intervention group. The rats were killed at 3, 7, 14 and 28 days after establis- hing model, fluorescent quantitation RT - PCR was used to detect the expression of Foxgl mRNA in cerebral tissues of the rats, the expres- sion of BrdU ＋ cells in hippocampal dentate gyrus was detected by immunohistochemical method, the expressions of BrdU ＋/Nestin ＋ , Br- dU ＋/GFAP ＋ and BrdU ＋/NSE ＋ cells in cerebral cortex cells were detected by double -labeling immunofluorescence. Results： The expres- sion levels of Foxgl mRNA, BrdU ＋ cells and BrdU ＋/Nestin ＋ cells at different time points from high to low were GB intervention group 〉 model group 〉 sham - operation group, there were statistically significant differences （ P 〈 0. 01 ） ; the expression level of BrdU ＋/NSE ＋ cells at different time points from high to low were GB intervention group 〉 model group 〉 sham - operation group, there was statistically significant difference （P 〈0. 01 ） ; the expression level of BrdU＋/GFAP＋ cells at different time points from high to low were GB intervention group 〉 model group 〉 sham - operation group, there was statistically significant difference （P 〈 0. 01 ） . Conclusion： GB can promote the prolifer- ation and differentiation of neural stem ceils, relieve and repair cerebral injury by up - regulating the expression of Foxgl mRNA in cerebral tissue of rats with HIBD.

关 键 词：缺氧缺血性脑损伤 银杏内酯B Foxg1mRNA 神经干细胞 

分 类 号：R722.1[医药卫生—儿科]