牦牛MC1R基因的多态性研究  

The Polymorphism of MC1R Gene in Yak

作　　者：陈思[1] 柴志欣[1,2] 王永[1,2] 钟金城[1,2] 

机构地区：[1]西南民族大学动物遗传育种学国家民委-教育部重点实验室,成都610041 [2]西南民族大学青藏高原研究院,成都610041

出　　处：《生物技术通报》2014年第4期87-90,共4页Biotechnology Bulletin

基　　金：国家科技支撑计划课题(2012BAD13B06);中央高校基本科研业务费专项资金项目(11NZYTH03)

摘　　要：旨在为探究牦牛MC1R基因多态性与毛色形成的相关性,利用PCR-SSCP和DNA测序技术,对64头牦牛(33头黑色九龙牦牛,31头白色天祝白牦牛)的MC1R基因多态性进行检测。结果表明:天祝白牦牛和九龙牦牛均有3种基因型(AA、BB、AB),但天祝白牦牛的多态性较低,而九龙牦牛表现为中度多态。经χ2适合性检验,2个牦牛品种在该基因多态位点上均偏离Hardy-Weinberg平衡。测序结果表明BB型与AA型在该片段的第179位碱基处存在C→A单碱基突变;第214位碱基处发生T→C突变。It was to explore the relationship between MC1R genotype and the coat color formation in yak, polymorphism of MC1R gene was detected by PCR-SSCP and DNA sequencing techniques in 64 yaks（33 white coat color and 31 black coat color）. The results showed that there were polymorphisms in the amplified region for the Tianzhu yak and Jiulong yak. In Tianzhu yak and Jiulong yak, three genotypes（AA, BB and AB）were detected. The result of χ2, three yak breeds were not fit for Hardy-Weinberg law. Sequencing revealed on single nucleotide mutation（C→A）at 179bp and（T→C）at 214 bp of the amplified region of Tianzhu yak and Jiulong yak.

关 键 词：牦牛 MC1R基因 PCR-SSCP 基因多态性 

分 类 号：S823[农业科学—畜牧学]