一株耐盐弧菌Vibrio sp.K1-L的分离与鉴定  

Isolation and Identification of a Halotolerant—Vibrio sp.K1-L

作　　者：王菲[1] 刘抗[1] 范家同[1] 成守亮[1] 王子元[1] 

机构地区：[1]江苏师范大学生命科学学院,徐州221116

出　　处：《生物技术通报》2014年第4期127-131,共5页Biotechnology Bulletin

基　　金：国家自然科学基金项目(30571071);江苏师范大学王子元教授科研启动基金(KY2009101);江苏高校优势学科建设工程项目;江苏省教育厅优秀创新团队项目

摘　　要：从山西运城盐湖水样中分离出一株细菌K1-L,能在2%-10%的MGM固体培养基上出现群游现象。该菌株为革兰氏阴性菌,周生鞭毛,电镜下大小约为0.4μm×(1-2)μm,其菌落形态为圆形,灰色。菌株K1-L的最适生长盐浓度、温度和pH值的范围分别为2%-6%、30-40℃和6.0-7.0,GC含量为47.06%。通过对其形态学、生理生化试验,16S rRNA基因序列及系统发育学的分析,发现其与Vibrio diabolicus HE800T和Vibrio rotiferianus LMG 21460T等细菌的16S rRNA有高度同源性,最大的相似性均为99%,确定该菌株K1-L为弧菌属(Vibrio)。Strain K1-L, isolated from Yuncheng Salt Lake, Shanxi Province, could swim on the 2%-10%MGM medium. The strain K1-L was Gram-negative with polar flagellum and was 0.4μm ×（1-2）μm under the scanning electron microscope. Strain K1-L appeared grey, rounded colony. Its optimum salt concentration, temperature and pH ranges were 2%-6%, 30-40℃and 6.0-8.0. The GC content is 47.06%. After comparing the traditional characteristics of morphology, physiological biochemistry and 16S rRNA sequence analysis, we discovered that the strain K1-L was highly homologous to the Vibrio diabolicus HE800T and Vibrio rotiferianus LMG 21460T. The maximum similarity was 99%. It was asserted that K1-L was belonged to the genus Vibrio, we proposed the name Vibrio sp. K1-L for this strain.

关 键 词：运城盐湖 弧菌属 耐盐菌 系统发育分析 

分 类 号：S917.1[农业科学—水产科学]