产耐酸性α-淀粉酶菌株的分离、鉴定、酶学特性研究及发酵培养基的优化  

Screening,Identification and Characterization of an Acid α-amylase Producing Strain and Optimization of Its Fermentable Condition

作　　者：王建玲[1] 陈志鑫[1] 刘逸寒[1] 路福平[1] 

机构地区：[1]天津科技大学生物工程学院工业发酵微生物教育部重点实验室,天津300457

出　　处：《生物技术通报》2014年第4期159-163,共5页Biotechnology Bulletin

基　　金：天津科技大学实验室开放基金项目(1204A211);国家自然科学基金资助项目(31101219);国家高技术研究发展计划("863"计划)项目(2013AA102106-07)

摘　　要：从富含淀粉的土壤中筛选获得多株产耐酸性α-淀粉酶的菌株,将酶活最高的一株经16S rDNA鉴定为枯草芽孢杆菌(Bacillus subtilis),命名为Bacillus subtilis A-7,其所产的耐酸性α-淀粉酶最适温度和pH分别为60℃和4.5。通过单因素筛选及正交试验优化发酵培养基,得到最佳配方为可溶性淀粉2%,蛋白胨2%,CaCl20.07%,Na2HPO40.8%,在此条件下耐酸性α-淀粉酶活力达到221 U/mL,是未优化条件下的2.3倍。Multiple new acidα-amylase producing strains were isolated from soil which is rich in starch. A strain which has the highest enzyme activity was identified as Bacillus subtilis A-7 through analysis of 16S rDNA gene. The optimum temperature and pH was 60℃and 4.5. The single factor experiments and orthogonal experiments were adopted to optimize the liquid fermentation medium. The composition of the best medium was 2%soluble starch, 2%peptone, 0.07%CaCl2, 0.8%Na2HPO4. Under this optimal condition, the activity of acidα-amylase was 221 U/mL and 2.3-fold more than that of no optimization.

关 键 词：耐酸性a-淀粉酶 16S rDNA鉴定 枯草芽孢杆菌 最适温度 最适PH 正交试验 

分 类 号：Q93[生物学—微生物学]