鼻咽癌患者血浆中S100A8和S100A9蛋白浓度测定及临床意义  

Determination of S100A8 and S100A9 protein in plasma of nasopharyngeal carcinoma patients and its clinical significance

作　　者：韩冉冉[1] 黄元姣[2] 陈铃[3] 肖晓兰[4] 易翔[5] 蔡红武[5] 吴勇浒[1] 

机构地区：[1]广西医科大学生物化学与分子生物学教研室,南宁530021 [2]广西医科大学医学科学实验中心,南宁530021 [3]广西医科大学附属肿瘤医院输血科,南宁530021 [4]广西医科大学附属肿瘤医院病案室,南宁530021 [5]广西医科大学第一附属医院耳鼻咽喉头颈外科,南宁530021

出　　处：《临床检验杂志》2014年第4期252-254,共3页Chinese Journal of Clinical Laboratory Science

基　　金：国家自然科学基金(81260405);广西壮族自治区自然科学基金(2011GXNSFA018233);广西区域性高发肿瘤早期防治研究重点实验室基金项目(GK2013-A-02-02)

摘　　要：目的探讨鼻咽癌患者血浆中S100A8和S100A9蛋白水平与临床分期及血浆EB病毒VCA-IgA抗体的相关性。方法用ELISA法检测鼻咽癌患者(Ⅱ期36例,Ⅲ期104例,Ⅳ期104例)和104例体检健康者血浆中S100A8、S100A9蛋白浓度和EB病毒VCA-IgA抗体水平,用SPSS 16.0统计软件进行数据分析。结果鼻咽癌患者血浆中S100A8、S100A9蛋白浓度分别为0.44(0.25,0.78)ng/mL和202.71(185.79,238.54)pg/mL,显著高于体检健康者0.29(0.18,0.44)ng/mL和192.43(170.66,193.42)pg/mL(P均<0.05)。Ⅱ、Ⅲ、Ⅳ期鼻咽癌患者血浆S100A8、S100A9蛋白浓度均高于体检健康者(P均<0.05),且Ⅱ、Ⅲ期鼻咽癌患者血浆中S100A8、S100A9蛋白浓度高于Ⅳ期(P<0.05);Ⅲ期S100A9蛋白浓度高于Ⅱ期(P<0.05)。相关分析结果显示,鼻咽癌患者血浆中S100A8、S100A9蛋白浓度与EB病毒VCA-IgA抗体水平无相关性(r分别为-0.04,-0.07,P均>0.05)。结论鼻咽癌患者血浆中S100A8和S100A9蛋白水平升高,且与临床分期有关。Objective To explore the correlation of the levels of $100A8 and S100A9 protein in the plasma of nasopharyngeal carcino- ma （NPC） patients with the clinical stages and the Epstein-Barr virus （EBV） VCA-IgA antibody. Methods ELISA method was used to examine the concentrations of S100A8 and S100A9 and the levels of EBV VCA-IgA in the plasma of NPC patients （ stage H ： 36 ca- ses, stage III ： 104 cases, stage IV ： 104 cases） and healthy subjects （ 104 cases）. The results were analyzed with SPSS 16.0 statisti- cal software. Results The concentrations of S100A8 and SI00A9 in the plasma of NPC patients were 0.44 （0.25, 0.78） ng/mL and 202.71 （185.79,238.54） pg/mL, which were significantly higher than those of healthy subjects 0.29 [ （0.18, 0.44 ） ng/mL and 192.43 （170.66, 193.42） pg/mL] （P 〈 0.05 ）. The concentrations of S100A8 and S100A9 in the plasma of NPC patients at stages II , II and IV were all higher than those of healthy subjects （ P 〈 0.05 ） , and those of stage H and m were higher than that of stage IV （ P 〈 0.05 ）. The concentration of S100A9 at stage 111 was higher than that of stage II （ P 〈 0.05 ）. No correlation between the concentrations of S100A8 and SIOOA9 and the levels of EBV VCA-IgA was found in the plasma of NPC patients （ r = - 0.04, - 0.07, all P 〉 0.05 ）. Conclusion The concentrations of S100A8 and SI00A9 protein in the plasma of NPC patients were elevated and asso- ciated with the clinical stages of NPC.

关 键 词：鼻咽癌 S100A8蛋白 S100A9蛋白 

分 类 号：R34[医药卫生—基础医学]