维生素D缺乏加剧细菌诱导的小鼠结肠炎的研究  

STUDY ON VITAMIN D DIFICIENCY TO WORSEN ULCERATIVE COLITIS INDUCED BY BACTERIA IN MICE

作　　者：李春艳[1] 王蕾[1] 张英[1] 钱艳[1] 邱服斌[1] 

机构地区：[1]山西医科大学公共卫生学院,太原030001

出　　处：《营养学报》2014年第2期141-144,共4页Acta Nutrimenta Sinica

摘　　要：目的研究维生素D(VD)缺乏对细菌诱导的小鼠溃疡性结肠炎的影响,并初步探讨其可能的机制。方法24只3w龄雌性C56BL/6小鼠随机分为阴性对照(NC)组,阳性对照(PC)组和VD缺乏模型(VDDM)组。建立VD缺乏模型后,用鼠伤寒沙门氏菌制备小鼠结肠炎模型。1w后,处死小鼠,分离结肠、肝、脾,称重。观察小鼠结肠组织大体形态,并进行组织学评分;ELISA法检测小鼠血清IL-17和IL-23的含量;RT-PCR法检测小鼠结肠组织维生素D受体(VDR)mRNA的表达。结果与NC组比,PC组和VDDM组体重下降,结肠湿重指数、肝重、脾重、组织学评分均增加,IL-17、IL-23及VDR表达增加;与PC组比较,VDDM组脾重和组织学评分增加,IL-17、IL-23蛋白的表达增加,但VDR基因的表达却降低。结论 VD缺乏影响VDR基因水平,加剧细菌诱导的小鼠溃疡性结肠炎,其机制可能与IL-23/IL-17炎性通路有关。Objective To investigate the effect of vitamin D （VD） deficiency on mice ulcerative colitis induced by bacteria and its possible mechanism. Methods Twenty-four 3-week-old C57BL/6 female mice were randomly divided into three groups： negative control （NC） group, positive control （PC） group and vitamin D deficient model （VDDM） group. After establishing VD deficient mice model, ulcerative colitis model was induced by Salmonella Typhimurium. One week later, all the mice were sacrificed and the colons, livers and spleens were separated and weighed. The macroscopic and histological scores of colon were evaluated. Serum levels of IL-17 and IL-23 protein were measured by ELISA, and vitamin D receptor （VDR） mRNA expression in colon was determined by RT-PCR. Results Compared with NC group, body weight in PC group and VDDM group were decreased, whereas the wet index and histological scores of colon tissues were increased, as well as liver weight, spleen weight, serum levels of IL-17 and IL-23, and VDR mRNA expression. The weight of spleen and histological scores of colon in VDDM group were increased compared with PC group, while the expressions of IL-17 and IL-23 were much higher. However, the expression of VDR mRNA was significantly lower than that in PC group. Conclusion VD deficiency may reduce the expression of VDR and aggravate ulcerative colitis induced by bacteria, which might be related with the alternation of IL-23/IL-17 pathway.

关 键 词：维生素D 溃疡性结肠炎 维生素D受体 IL-17 IL-23 

分 类 号：R591.44[医药卫生—内科学]