叶酸对小鼠甲基代谢作用的研究  

STUDY ON THE EFFECT OF FOLIC ACID ON METHYL GROUP METABOLISM IN MICE

作　　者：秦善春[1] 刘欢[1] 赵世晶[1] 张大龙[1] 赛娜[1] 黄国伟[1] 

机构地区：[1]天津医科大学公共卫生学院营养与食品卫生学系,天津300070

出　　处：《营养学报》2014年第2期145-148,共4页Acta Nutrimenta Sinica

基　　金：国家自然科学基金(No.81130053;81202200)

摘　　要：目的研究叶酸缺乏和补充对小鼠甲基代谢的作用。方法小鼠随机分为叶酸缺乏组(FA-D)、叶酸对照组(FA-C)和叶酸补充组(FA-S)三组,叶酸干预4 w,采用化学发光免疫法检测血清叶酸浓度;高效液相色谱法检测蛋氨酸循环代谢产物同型半胱氨酸(homocysteine,Hcy)、S-腺苷甲硫氨酸(S-adenosylmethionine,SAM)、S-腺苷高半胱氨酸(S-adenosylhomocysteine,SAH)在血浆、脑组织和肝脏中的含量。结果叶酸缺乏组血浆、脑组织和肝脏的SAH含量高于对照组,SAM/SAH比值低于对照组(P<0.05);叶酸补充组SAH含量低于对照组,而SAM/SAH比值高于对照组(P<0.05)。叶酸缺乏组和叶酸补充组血浆Hcy则分别高于和低于对照组(P<0.05)。结论叶酸缺乏可降低SAM/SAH比值,使体内甲基供体水平下降,影响小鼠正常甲基代谢;而叶酸补充可以提供相对更多甲基供体SAM,降低小鼠Hcy水平。Objective To explore the effects of folic acid deficiency and supplementation on methyl group metabolism in mice. Methods Mice were randomly divided into folic acid deficiency group（FA-D）, folic acid control group（FA-C） and folic acid supplementation group（FA-S）. Mice were treated with different doses of folic acid for 4 weeks. Serum folate concentration was detected by chemiluminescent immunoassay. Homocysteine（Hcy）, S-adenosylmethionine （SAM） and S-adenosylhomocysteine（SAH） levels in plasma, brain and liver were detected by high performance liquid chromatography. Results SAH concentration in plasma, brain and liver in FA-D group was higher than in FA-C group. SAM/SAH ratio in FA-D group was lower than in FA-C group. SAH concentration in FA-S group was lower than in FA-C group （P〈0.05）. SAM/SAH ratio in FA-S group was higher than in FA-C group（P〈0.05）. Folic acid deficiency could lead to higher plasma Hcy, but folic acid supplementation could decrease plasma Hcy levels （P〈0.05）. Plasma Hcy level in FA-D group and FA-S group was higher and lower than in FA-C group respectively（P〈0.05）. Conclusion Folic acid deficiency can reduce SAM / SAH ratio, decrease the methyl donor level and inhibit normal methyl group metabolism in mice. Folic acid supplementation can provide relatively more methyl donor SAM and reduce Hcy level in mice.

关 键 词：叶酸 甲基代谢 S-腺苷甲硫氨酸 S-腺苷高半胱氨酸 同型半胱氨酸 

分 类 号：R591.424[医药卫生—内科学]