不同肥胖易感性大鼠模型的建立及其能量代谢的研究  

ESTABLISHMENT OF OBESITY PRONE AND OBESITY RESISTANT RAT MODELS AND THEIR METABOLIC STUDY

作　　者：刘健敏[1] 王俊霞[1] 郑龙[1] 连伟光[1] 徐增年[1] 刘树锋[1] 

机构地区：[1]河北医科大学实验动物重点实验室,石家庄050017

出　　处：《营养学报》2014年第2期184-187,共4页Acta Nutrimenta Sinica

基　　金：河北省科学技术研究与发展计划项目(No.11277170);河北医科大学科研骨干人才培育计划资助项目(No.30900143)

摘　　要：目的建立不同肥胖易感性大鼠动物模型,并探讨其能量代谢的特点。方法雄性SD大鼠60只,给予高脂饲料喂养3 w后,按体重增加量排序,上1/3(18只)作为肥胖易感(OP)组,下1/3(18只)作为肥胖抵抗(OR)组,中间1/3作为对照组。继续喂养12w后,每组随机挑选一半动物,每日给予半量饲料进行限食饲喂,另一半继续自由采食,持续2 w后,测量肥胖易感(OP)、肥胖抵抗(OR)及对照组大鼠不同状态下能量摄入量、基础代谢率(BMR)、静息代谢率(RMR)、基础能量消耗(BEE)等代谢指标。结果 OP大鼠体重、Lee’s指数、BMI指数、能量利用率均显著高于OR组和对照组,OR组与对照组之间无显著差异。在自由采食状态下,OP、OR和对照组间能量摄入水平无显著差异,OR组BMR、RMR及BEE水平显著高于对照组和OP组。在半量限食状态下,各组代谢水平较自由采食状态均有所降低,OR组BMR、RMR及BEE水平较自由采食状态降低幅度最大。结论 OP和OR大鼠在基础代谢方面存在差异,且OR较OP具有更为灵敏的能量平衡调节系统。Objective To establish obesity prone （OP） and obesity resistant （OR） rat models by feeding a high-fat diet （HFD）, and investigate their energy metabolic characteristics. Methods Sixty male Sprague Dawley （SD） rats were fed a high fat diet for 3 w, and then the OP and OR rats were selected according to their body weight gains. The rats with the most weight gain（first tertile） during this period of time were identified as obesity prone （OP） and the third tertile were identified as obesity resistant （OR）, and the remaining as control group. All the rats were fed their respective diets for another 12 w for development of obesity. Then, half of the rats in each group （OP, OR and Control） were placed on a food restriction schedule and were allowed access to 50% of their individual baseline mean daily food intake each day, while the other half were maintained ad libitum on food for 2 w. The changes of body weight, body length, energy intake （EI）, basal metabolic rate （BMR）, resting metabolic rate （RMR） and basal energy expenditure （BEE） were measured. Results After 15w feeding, OP rats had significantly higher （P〈 0.01） body weight, Lee＇s index, BMI and energy efficiency than those in other two groups. There was no significant differences between OR and control group in body weight. In the ad libiturn feeding state, no difference （P〉0.05） was found in EI. BMR, RMR and BEE in OR were significantly higher （P〈0.05） than in other two groups. Diet restriction led to a reduction in BMR, RMR and BEE in all groups. OR rats showed a significantly greater reduction （P〈0.05）. Conclusion There are significant differences between OP and OR rats in BMR either in HFD ad libitum condition or diet restricted feeding state. OR rat has the ability to sense and respond to energy imbalance accurately while the ability in OP is blunted.

关 键 词：肥胖易感 肥胖抵抗 能量摄入 能量消耗 

分 类 号：R589.2[医药卫生—内分泌] R151.4[医药卫生—内科学]