云南鼠疫菌Asc I酶切分型及其流行病学意义  

Genotyping of Yunnan Yersinia pestis by Asc I enzyme digestion and its epidemiological significance

作　　者：叶蕊[1] 石丽媛[1] 王鹏[1] 董珊珊[1] 郭英[1] 杨光璨[1] 崔志刚[2] 李伟[2] 宋志忠[1] 

机构地区：[1]云南省地方病防治所云南省鼠疫防控技术重点实验室,云南大理671000 [2]中国疾病预防控制中心传染病预防控制所传染病预防控制国家重点实验室,北京昌平102206

出　　处：《疾病预防控制通报》2014年第2期1-3,23,共4页Bulletin of Disease Control & Prevention(China)

基　　金：国家自然科学基金(81160354);卫生部行业基金(201202021);云南省应用基础研究(2011FB134)

摘　　要：目的构建以AscI酶切的云南省鼠疫菌PFGE图谱库并探究其流行病学意义。方法采用限制性内切酶AscI对云南家、野鼠型及玉龙鼠疫菌进行酶切分析，并对电泳图谱进行聚类分析。结果30株被试菌株分为15种PFGE型别（相似性系数为88．9％～100．0％）、5个簇、4个群，除404号菌与EV76归为一群，其余3个群分别为家鼠型基因群、野鼠型基因群及玉龙基因群。结论云南鼠疫菌PFGE基因型与生态型相吻合，具有一定的区域聚集性；玉龙鼠疫菌是云南独立的一个基因群。Objective To create fingerprint library of Yunnan Yersinia pest＆ by pulse field gel electrophoresis （PFGE） with Asc I enzyme digestion and to study its epidemiological significance. Methods We used rare cutting restriction en- zyme Asc I to digest Yunnan E pestis strains isolated from Rattusflavipectus plague focus, .4podemus chevrieri-Eothenomys miletus plague focus and Yulong plague focus, and submitted fingerprints to Bionumerics software for cluster analysis. Re- sults Thirty tested strains were divided into 15 genotypes with 88.9%-100.0% similarity value. 15 genotypes formed 4 groups of 5 clusters, one cluster consisted of No.404 and EV76, the other three were of Rattus flavipectus plague group, Apodemus chevrieri-Eothenomys miletus plague group and Yulong group, respectively. Conclusions PFGE genotypes of Yunnan Y pestis accord with their ecotypes, and have cluster regional feature. The strains isolated from Yulong, are of a unique PFGE type, indicating a new clone group.

关 键 词：鼠疫耶尔森菌 脉冲场凝胶电泳 ASC I 

分 类 号：R516.8[医药卫生—内科学]