RDP-p53融合蛋白对小鼠炎症细胞因子的影响  

Effects of RDP-p53 Fusion Protein on Inflammatory Cytokine of Mice

作　　者：赵博[1] 孙梅[1] 刘婷[1] 赖利金 付爱玲[1] 徐兴然[1] 

机构地区：[1]西南大学药学院,重庆400715

出　　处：《科学技术与工程》2014年第10期1-3,10,共4页Science Technology and Engineering

基　　金：国家自然科学基金(81273416);国家大学生创新实验计划(201210635075)资助

摘　　要：研究不同剂量RDP-p53融合蛋白对小鼠炎症细胞因子的影响。通过大肠杆菌Rosetta表达蛋白并纯化,SDS-PAGE确定RDP-p53融合蛋白准确性。同时,将昆明小鼠分为空白对照组和RDP-p53融合蛋白高、中、低剂量组,经腹腔注射给药,摘眼球取血,用酶联免疫吸附法(ELISA)测定血清中白细胞介素1β(IL-1β)、白细胞介素6(IL-6)和肿瘤坏死因子α(TNF-α)的含量。RDP-p53融合蛋白在上清和沉淀中均获得表达。ELISA测定结果表明,与对照组比较,低、中剂量组小鼠血清炎症因子水平没有明显变化(P>0.05),高剂量组的IL-1β和TNF-α含量显著升高(P<0.05)。成功制备RDP-P53融合蛋白,并为进一步研究RDP-p53融合蛋白抗神经胶质瘤药理作用时给药剂量的确定提供了实验依据。The effects of different doses of RDP-p53 fusion protein in treatment mice g inflammatory cytokine is studied. RDP-p53 fusion protein was expressed by E. coli Rosetta and got purified, the expressed product was identi- fied by SDS-PAGE. Then Kunming mice were divided into 4 groups, i. e. blank control group and RDP-p53 fusion protein high-dose,medium-dose and low-dose groups. Kunming mice were immunized by peritoneal route, of which the sera were separated and determined for IL-1 β ,IL-6 and TNF-a level by ELISA. RDP-p53 fusion protein got ex- pression in both supernatant and precipitation. ELISA results showed that：compared with control group, inflammato ry cytokine content of low-dose and medium-dose groups didn increase significantly（P 〉0.05 ） ,IL-Iβ and TNF-acontent of high-dose groups increased significantly（ P 〈 0.05 ）. RDP-p53 fusion protein was successfully prepared, this might provide some valuable information for determining dosage of administration while studying pharmacologi-cal effect that RDP-p53 fusion protein inhibits brain tumors.

关 键 词：P53 炎症细胞因子 细胞穿膜肽 

分 类 号：R963[医药卫生—微生物与生化药学]