环磷酰胺及长春新碱对睾丸支持细胞间隙连接蛋白表达的影响  

Influence of Cyclophosphamide and Vincristine on the Expression of Connexin 43 of Sertoli Cells

作　　者：侯高铭 郑俊鸿[1] 周德荣[1] 杨庆涛[1] 

机构地区：[1]汕头大学医学院第二附属医院泌尿外科,广东汕头515041

出　　处：《汕头大学医学院学报》2014年第1期24-26,32,F0002,共5页Journal of Shantou University Medical College

基　　金：广东省科技计划资助项目(2011B080701090)

摘　　要：目的:探讨环磷酰胺、长春新碱对睾丸支持细胞间隙连接蛋白(Cx)43表达的影响。方法:培养小鼠睾丸支持细胞,分别加入环磷酰胺(0、1、2、4、8μg/mL)和长春新碱(0.00、0.01、0.02、0.04、0.08μg/mL)行细胞毒染24 h,MTT法检查细胞毒性。选择4μg/mL环磷酰胺、0.04μg/mL长春新碱作用支持细胞6、12、24及48 h,结果行RT-PCR分析;选择同样浓度药物作用支持细胞24 h,并用免疫荧光染色检测培养的细胞中Cx43的表达情况。结果:环磷酰胺、长春新碱染毒细胞后,Cx43 mRNA水平开始随时间增加而逐渐下降(P<0.05),且随剂量增加Cx43表达强度逐渐减弱(P<0.05)。结论:环磷酰胺及长春新碱对睾丸支持细胞有毒性,并随着药物浓度增大毒性增强,且环磷酰胺比长春新碱更明显。Objective：To discuss the influence of cyclophosphamide and vincristine on the expression of connexin（Cx）43 of sertoli cells. Methods：Sertoli cells were cultured and treated with 0,1,2,4,8 μg/mL cyclophosphamide and 0.00, 0.01,0.02,0.04,0.08 μg/mL vincristine. The cytotoxicity of cyclophosphamide and vincristine was assessed by MTT assay. Cyclophosphamide（4 μg/mL）and vincristine（0.04 μg/mL）were selected to act on sertoli cells 6,12,24 and 48 hours,then the result analysis by RT-PCR was carried on. Sertoli cells were cultured in the same concentration of cyclophosphamide and vincristine for 24 hours,then the expression of Cx43 in sertoli cells was detected by immunofluorescence staining. Results： The expression of Cx43 in sertoli cells decrease gradually with the prolonging of time（P0.05） and the increasing of drug concentration（P0.05）. Conclusion：Cyclophosphamide and vincristine have toxicity on sertoli cells,and with the increase of drug concentration the toxicity will enhance. Cyclophosphamide has more toxicity than vincristine.

关 键 词：环磷酰胺 长春新碱 睾丸支持细胞 间隙连接蛋白43 

分 类 号：R169.41[医药卫生—公共卫生与预防医学] R339.21