鸡胚分离不同型别流感病毒的敏感性分析  

Analysis of sensitivities of virus isolation for different types of influenza by chicken embryonated eggs method

作　　者：杨式芹[1] 翁育伟[1] 洪荣涛[1] 谢剑锋[1] 黄萌[1] 张炎华[1] 郑奎成 

机构地区：[1]福建省人兽共患病研究重点实验室(福建省疾病预防控制中心),福州350001

出　　处：《海峡预防医学杂志》2014年第2期1-3,共3页Strait Journal of Preventive Medicine

基　　金：国家科技重大专项(No.2012ZX10004-210)

摘　　要：目的了解不同流感病毒型别鸡胚培养的敏感性及分离毒株的生物学特征。方法将流感病例呼吸道标本核酸检测阳性者接种SPF鸡胚,用红血球凝集试验判定分离结果并测定病毒滴度,以血凝抑制试验鉴定毒株型别。结果 802份核酸阳性标本鸡胚分离阳性536份,阳性率66.8%,其中A(H1N1)、A(H3N2)、B和H1N1pdm2009的阳性率分别是52.3%(45/86)、4.3%(3/70)、62.4%(194/311)和87.8%(294/335);首次出现阳性的血凝滴度分别是1∶18.92、1∶15.96、1∶18.83和1∶5.93;合格(血凝滴度≥1∶8)阳性培养物传代数分别为2、2、2和3代。结论鸡胚是流感病毒A(H1N1)和B型的敏感宿主,连续传代有利于提高流感病毒的分离率和血凝滴度。Objective To analyze the sensitivities for influenza virus isolation by chicken embryonated eggs method and biological characteristics of isolated strains.Methods Respiratory specimens from influenza like illness（ILI） patients were collected and specimens positive for viral nucleic acid amplification tests were inoculated into specific-pathogen-free（SPF） chicken embryonated eggs,virus titers were detected by hemagglutination tests （HA） and subtyped by hemagglutination inhibition tests（HI）. Results The positive rates for influenza A（H1N1）,A（H3N2）,B and H1N1pdm2009 viruses were 52.3%（45/86）,43%（3/70）,62.4%（194/311） and 87.8%（294/335）,respectively.The initial HA mean geometric titers were 1：18.9,1：15.9,1：18.8 and 1：5.9,respectively.However,additionally another 2 or 3 passages in embryonated eggs were necessary to increase HA titers to over 1：8,as demanded by virus typing with HI method.Conclusion Chicken embryonated eggs were sensitive hosts for influenza A（H1N1） and B virus,and that continuous passages in chicken embryonated eggs could increase positive rates and HA titers for several types of influenza virus.

关 键 词：流感病毒 病毒分离 鸡胚法 敏感性 血凝滴度 

分 类 号：R373.1[医药卫生—病原生物学]