发根农杆菌Ri质粒转化康乃馨初步研究  

A PRELIMINARY STUDY ON CARNATION BY RI PLASMID OF Agrobacterium rhizogenes

作　　者：范惠琴[1] 姚泉洪[1] 彭日荷[1] 黄晓敏[1] 李贤[1] 

机构地区：[1]上海市农业遗传育种重点实验室,上海市农业科学院生物技术研究中心,上海201106

出　　处：《上海农业学报》2001年第1期35-38,共4页Acta Agriculturae Shanghai

基　　金：上海市科技发展基金科技攻关项目!(编号 :9739130 11)

摘　　要：利用具有Ri质粒的发根农杆菌 (Agrobacteriumrhizogenes)Pri15834对康乃馨组培苗的带叶茎节进行转化试验。转化后的外植体在K0 附加 50 0mg/L羧苄青霉素培养基上培养 2～ 3周后 ,受感染的伤口部位长出无向地性的毛状根 ,频率为 37.0 7%。将毛状根切碎分别在K0 、K1、K2 培养基上继续培养 3周 ,毛状根在K0 上表现为无限生长 ,在K1上主要表现为长愈伤组织 ,频率为 51.4 2 % ,而在K2 上则主要表现为长绿苗 ,频率为 2 5%。Using Ri plasmid of Agrobacterium rhizogenes (Pri15834), the carnation transformation experiment with leafed stem segments of shoot from tissue culture as initial material was carried out. After transformation, explants were transferred to K 0 medium with 500mg/L carbenicillin and cultured for 2～3 weeks, then non geotropic hairy roots formed from infected wound sites at a frequency of 37.07%. Afterwards,the hairy roots were cut up and subcultured on K 0,K 1,K 2 mediums with 250mg/L carbenicillin for 3 weeks. The results of subculture showed that the hairy roots on K 0 medium grew indefinitely, those on K 1 medium formed calli at a frequency of 51.42%, those on K 2 medium formed green plantlets at a frequency of 25%, indicating expression of Ri plasmid integrated into the genome of carnation cell.

关 键 词：康乃馨 发根农杆菌 RI质粒 毛状根 转化 

分 类 号：S681.5[农业科学—观赏园艺]