A蛋白酶联免疫吸附法检测葡萄卷叶病毒的研究用离体微嫁接法快速检测苹果潜隐病毒  

Detection of grapevine leaf-roll virus by PAS-ELISA

作　　者：何水涛[1] 周厚成[1] 刘崇怀[1] 卢其真[2] 

机构地区：[1]中国农业科学院郑州果树研究所,郑州450009 [2]河南农业大学园艺系

出　　处：《落叶果树》2001年第2期1-4,共4页Deciduous Fruits

基　　金：河南省科技厅重大科技攻关项目

摘　　要：研究了用A蛋白酶联免疫吸附检测法 (PAS -ELISA)检测葡萄卷叶病毒的程序 ,提出了A蛋白、酶标A蛋白、抗体的适宜工作浓度及样品粗提液稀释倍数。通过对感病植株的幼叶、幼茎、老叶、老茎的检测研究 ,规范了PAS -ELISA检测技术。应用该技术 ,对国家果树种质资源圃郑州葡萄圃的 39份种质及郑州地区栽培的品种进行了检测 ,保存品种的带毒率 76 .9% ,抗逆性较强的东亚种群带毒率 55.6 % ,郑州地区栽培品种葡萄带毒率为 4 5.0 %。The suitable concentration of protein-A,enzyme-labelled protein-A and antibody,and the dilution multiple of sample crude extract were ascertained in detecting grapevine leaf-roll virus with young leaves and old leaves and young stems and old stems by PAS-ELISA. Detection of 39 germ plasms in the Zhengzhou Grapevine Garden of the Chinese Pomology Germplasm Gardens and cultivated grapevines in Zhengzhou Area by PAS-ELISA showed that the virused rate was 76.9% for the varieties preserved in Zhengzhou Grapevine Graden, 55.6% for the Eastern Asian species, and 45.0% for the varieties cultivated in Zhengzhou Area.

关 键 词：葡萄病毒 PAS-ELISA 葡萄卷叶病毒 检测方法 

分 类 号：S436.631.1[农业科学—农业昆虫与害虫防治]