Random Amplified Polymorphic DNA （RAPD） Analysis on Blumeria graminis f.sp. tritici Isolates Collected in Central Gansu Province, China  

作　　者：Long WANG Li-Ping GAN Sheng-Rong WANG 

机构地区：[1]Department of Plant Protection, College of Grassland, Gansu Agricultttral University Lanzhou, 730070, China [2]Department of Biology, Chongqing Three Gorges University. Wanzhou, 404000, China [3]Institute of Biology, Gansu Academy of Sciences. Lanzhou,730000, China

出　　处：《International Journal of Technology Management》2014年第5期25-28,共4页国际技术管理

摘　　要：Twenty isolated strains of Blumeria graminis f.sp. tritici collected from central Gansu province were studied with random amplified polymorphic DNA （RAPD） analysis. PCR amplifications using nine random primers generated a total of 81 bands, of which, 54 were polymorphic. The total percentage of polymorphic bands varied from 50.0% to 88.9%. The average percentage based on RAPD patterns was approximately 66.7%, which indicated high heredity differentiation among isolates. Clustering analysis showed that the polymorphism of the twenty isolates was related to geographical origins but had little relationship with the physiological race.

关 键 词：Blumeria graminis f.sp. tritici Physiological race RAPD Polymorphic frequency 

分 类 号：Q959.468[生物学—动物学] Q943