高压氧预处理对减压病大鼠脑组织B细胞淋巴瘤/白血病基因-2蛋白及相关X蛋白表达的影响  

Effect of hyperbaric oxygen preconditioning on the expressions of B cell lymphoma/lewkmia-2 and Bcl-2 associated X protein in the brain tissue of rats with decompression sickness

作　　者：李娅[1] 岳荣[2] 王文岚[1] 薛莉[1] 任杰[1] 谢小萍[1] 迪力达尔[2] 李金声[1] 

机构地区：[1]第四军医大学航空航天医学院卫生与卫生勤务学教研室,西安710032 [2]新疆维吾尔自治区人民医院高压氧科

出　　处：《中华航海医学与高气压医学杂志》2014年第1期1-6,共6页Chinese Journal of Nautical Medicine and Hyperbaric Medicine

基　　金：陕西省自然科学基础研究计划项目（2013JM4004）

摘　　要：目的 探讨高压氧（hyperbaric oxygen,HBO）预处理对减压病大鼠脑组织B细胞淋巴瘤/白血病基因-2蛋白（B cell lymphoma/lewkmia-2,Bcl-2）及相关X蛋白（Bcl-2 associated X protein,Bax）、半胱氨酸天冬氨酸蛋白酶-3蛋白（cysteine aspartic acid specific protease-3,Caspase-3）表达的影响.方法 健康雄性SD大鼠72只,采用数字表法随机分为正常对照组（对照组）、高压氧预处理组（HBO组）、减压病组（DCS组）,每组分别设置4个观察时间点（1、5、7、10 d）,每个时间点（亚组）6只.建立大鼠减压病模型,HE染色观察减压病后脑组织病理变化,免疫组织化学染色观察各组脑组织Bcl-2、Bax、Caspase-3 的表达.结果 （1） HBO组与DCS组脑组织皮层病情分级为轻度～中度（1～3级）.（2）HE染色发现,DCS组大鼠大脑皮层区出现大片疏松区,皮层与海马神经元细胞呈三角形变性坏死,细胞萎缩、体积缩小变性,染色质浓缩甚至碎裂,HBO组神经元变性坏死明显减轻.（3）1、5、7d时,DCS组脑组织皮层Bcl-2阳性细胞数分别为（89.5±15.60）、（176.4±10.22）、（265.52±15.74）个,与对照组（408.67 ±29.57）个相比明显减少,差异均有统计学意义（P＜0.01）;HBO组[脑组织皮层阳性细胞数分别为（179.64±12.21）、（253.91±14.00）、（341.15±13.52）个],较DCS组明显增加,差异均有统计学意义（P＜0.05）.1、5、7d时,DCS组脑组织皮层Bax阳性细胞数分别为（389.56±18.62）、（337.04±14.85）、（176.41±20.75）个,Caspase-3阳性细胞数分别为（495.64 ±21.03）、（283.04±13.12）、（352.41 ±21.34）个,较对照组1d时[脑组织皮层Bax和Caspase-3分别为（98.64±14.25）、（106.20±14.64）个]明显增加.HBO组Bax阳性细胞数分别为（313.54±21.02）、（253.05±13.60）、（129.03±12.85）个,Caspase-3分别为（429.43±14.08）、（228.05 ±13.60）、（301.02±15.79）个,较DCS组明显减少,差异均有统计学意义（P�Objective To investigate the effect of hyperbaric oxygen （HBO） preconditioning on the expressions of B cell lymphoma/lewkmia-2 （Bcl-2）,Bcl-2 associated X protein （Bax） and cysteine aspartic acid specific protease-3 （Caspase-3） in the brain tissue of rats with decompression sickness.Methods Seventy-two male healthy Sprague-Dawley rats were randomly divided into the normal control,the HBO preconditioning group （or HBO group）,the decompression sickness group （or the DCS group）.Four time points （at days 1,5,7 and 10） were set for each subgroup,which consisted of 6 animals.Pathological changes in the brain tissue,following development of the decompression sickness model,were observed with HE staining,and the expressions of Bcl-2,Bax and Caspase-3 were detected with immunohistochemistry in various subgroups.Results （1） For the animals in the HBO and DCS groups,the state of illness was classified as mild and moderate （grade 1-3）.（2） HE staining revealed that a large loose area in the rat cerebral cortex of the DCS group was detected,cortical and hippocampus neurons displayed a triangular degeneration and necrosis,cellular atrophy,volume reduction and degeneration,chromatin condensation or even fragmentation.For the animals in the HBO group,necrotic neuron degeneration and necrosis obviously alleviated.（3） At days 1,5 and 7,the expressions of Bcl-2 positive cells for the DCS group were （89.5 ± 15.60）,（176.4 ± 10.22）and （265.52± 15.74） respectively,which were significantly lower than those in the control group （408.67 ± 29.57）,with statistical significance （P ＜ 0.05）,while on the other hand,the expressions of Bcl-2 positive cells for the HBO group were （179.64 ± 12.21）,（253.91 ± 14.00） and （341.15 ± 13.52） respectively,which were significantly higher than those of the DCS group,also with statistical significance （P ＜ 0.05）.At day 1,5 and 7,the expressions of Bax positive cells for the DCS group were （389.56 ± 18.62）,（337.04 �

关 键 词：减压病 高压氧 B细胞淋巴瘤 白血病基因-2蛋白 Bcl-2蛋白相关X蛋白 半胱氨酸天冬氨酸蛋白酶-3蛋白 

分 类 号：R459.6[医药卫生—治疗学]