减压病兔脑和脊髓组织损伤机制及高压氧疗效  

Mechanism of brain and spinal cord tissue injury following decompression sickness in experimental rabbits and the effect of hyperbaric oxygen

作　　者：王翠翠[1] 刘晓红[1] 毛蕊琪[1] 曹永成[1] 毕利泉[1] 耿明[1] 

机构地区：[1]济南军区总医院病理科,济南250031

出　　处：《中华航海医学与高气压医学杂志》2014年第1期7-10,共4页Chinese Journal of Nautical Medicine and Hyperbaric Medicine

基　　金：全军医学科研“十二五”面上项目资助课题（CWS11J241）

摘　　要：目的 探讨实验性减压病兔脑和脊髓组织损伤机制及高压氧（hyperbaric oxygen,HBO）的治疗作用.方法 成年健康雄性新西兰大耳白兔28只,按数字表法随机分为正常对照组、减压病（DCS）组、安全减压组、HBO组,每组7只.DCS组,5 min空气加压至0.8 MPa（绝对压）,停留60 min,5 min匀速减至常压;HBO组按DCS组操作,出舱后立刻用加压舱行HBO治疗;安全减压组参考我国海军空气潜水减压表减至常压.动物出舱后30 min内迅速处死,取各组胸腰段脊髓和脑,借助光镜观察脊髓和脑的病理形态学改变,用原位末端脱氧核糖核酸转移酶介导DUTP标记法（TUNNEL法）观察胸腰段脊髓和脑组织凋亡情况.结果 DCS组脊髓白质内空泡形成,损伤区周围胶质细胞增生;TUNNEL标记阳性凋亡细胞数为每高倍镜视野下（28.29 ±2.56）个,较正常对照组（0.57±0.54）个、安全减压组（2.29±1.11）个、HBO组（3.71±1.1 1）个均明显增多,差异均有统计学意义（P ＜0.05）;HBO组阳性凋亡细胞数较正常对照组多,但较DCS组明显减少,差异均有统计学意义（P ＜0.05）;HBO组与安全减压组阳性细胞数差异无统计学意义（P ＞0.05）;DCS组脑组织凋亡细胞数[（1.43±0.54）个]较脊髓组织中凋亡细胞数[（28.29 ±2.56）个]明显减少,但多于正常对照组,差异具有统计学意义（P＜0.05）.结论 脊髓是减压病中枢神经系统损伤的主要部位;细胞凋亡是减压病脊髓损伤的原因之一;高压氧治疗能明显抑制大鼠脊髓损伤后的细胞凋亡,对减压病有良好的治疗作用.Objective To investigate the mechanism of brain and spinal cord injury in experimental rabbits with decompression sickness （DCS） and the effect of hyperbaric oxygen （HBO）.Methods Twentyeight adult male healthy New Zealand rabbits were randomly divided into 4 groups：the normal control group,the DCS group,the safe decompression group and the HBO group,each consisting of 7 animals.The rabbits in the DCS group were compressed with air in 5 minutes to a pressure 0.8 MPa and maintained at the said pressure for 60 min,and then were decompressed to normal pressure within 5 min.The animals in the HBO group underwent the same compression and decompression procedures,as the animals in the DCS group,but were immediately put into the hyperbaric chamber to receive HBO therapy.The safe decompression group was compressed and decompressed with the profile of the Chinese Navy Diving Decompression Tables.Changes in pathological morphology and apoptosis of brain and spinal cord tissue cells in the thoracic and lumbar segments were observed with light microscopy and terminal deoxynucleotidyl transferase-mediated DUTP nick end labeling （TUNEL） method.Results For the animals in the DCS group,cavity in the white matter of the spinal cord and proliferation of gliocytes around the damaged tissue could obviously be observed.TUNEL results showed that more positive apoptotic cells were detected in the DCS group （28.29 ± 2.56）/HP,as compared with those of the normal control group （0.57 ± 0.54）/HP,the safe decompression group （2.29 ± 1.11）/HP and the HBO group （3.71 ± 1.11）/HP,with statistical significance （P ＜ 0.05）.More positive apoptotic cells were also seen in the HBO group （3.71 ± 1.1 1）/HP,as compared with those of the normal control group （P ＜0.05）,but were significantly less,as compared with those of the DCS group （P ＜ 0.05）,also with statistical significance （P ＜ 0.05）.Slightly more positive apoptotic cells were detected in the animals of the HBO group,when compared with t

关 键 词：减压病 脊髓损伤 脑损伤 细胞凋亡 胶质细胞 高压氧 兔 

分 类 号：R852.16[医药卫生—航空、航天与航海医学]