高压氧对脑缺血再灌注小鼠肺组织白细胞介素-1β和白细胞介素-10水平的影响  

Effects of hyperbaric oxygen on the level of interleukin-1β and interleukin-10 in the lung tissue of mice following cerebral ischemia-reperfusion

作　　者：雷箴[1] 贾兴元[1] 王国忠[2] 

机构地区：[1]首都医科大学附属北京朝阳医院医学研究中心,北京100020 [2]民航总医院高低压氧科

出　　处：《中华航海医学与高气压医学杂志》2014年第1期15-17,共3页Chinese Journal of Nautical Medicine and Hyperbaric Medicine

摘　　要：目的 观察高压氧（hyperbaric oxygen,HBO）对脑缺血再灌注（ischemia-repeffasion,IR）小鼠肺组织白细胞介素-1β（IL-1β）和白细胞介素-10（IL-10）水平的影响,探讨其作用和意义.方法 63只成年雄性BALB/c小鼠按抽签法随机分成3组,每组又分为3个亚组,分别为：（1）IR组：1R4h组、IR24h组、IR48h组;（2） HBO组：IR4h±HBO组、IR24h±HBO组、IR48h±HBO组;（3）假手术（sham）组：sham4h组、sham24h组、sham48h组.每个亚组7只.夹闭小鼠双侧颈总动脉,建立脑缺血再灌注动物模型.采用ELISA法检测缺血再灌注4 h（IR4h）、24 h（IR24h）和48 h（IR48h）时各组小鼠肺组织IL-1β和IL-10水平.结果 在IR4h时,HBO组[（217.50 ±40.45） pg/g]和IR组[（250.15 ±45.61） pg/g] IL-1β水平均显著高于同时间点sham组[（139.17±31.49）pg/g]（均为P＜0.01）;HBO组和IR组IL-1β水平差异无统计学意义.在IR24h时,HBO组[（189.48±30.18） Pg/g]和IR组[（235.86±46.87） pg/g]IL-1β水平均显著高于同时间点sham组[（137.88±33.54） Pg/g]（分别为P＜0.05,P＜0.01）,HBO组IL-1β水平显著低于同时间点IR组（P＜0.05）;HBO组IL-10水平显著高于IR组和sham组（均为P＜0.05）.结论 HBO可显著降低IR小鼠肺组织IL-1β水平、升高IL-10水平,对IR所致急性肺损伤具有一定的保护作用.Objective To observe the effects of hyperbaric oxygen （HBO） on the level of IL-1β and IL-10 in the lung tissue of mice following cerebral ischemia-reperfusion,and also to investigate their role and significance.Methods Adult male BALB/c mice were randomly divided into the ischemia-reperfusion group （or the IR group）,the HBO group and the sham group.Cerebral ischemia-reperfusion model was established by clamping bilateral carotid arteries of mice.Level of IL-1β and IL-10 in the lung tissue at h 4,24 and 48 were measured respectively after cerebral ischemia-reperfusion by ELISA.Results The levels of IL-1β in the lung tissue of mice in the hyperbaric oxygen group and the ischemia-reperfusion group at h 4 were significantly higher than that of the sham group at the same time point （P ＜0.01）.No statistical significance in the IL-1β levels could be seen in the lung tissue of mice between the HBO group and the ischemia-reperfusion group.The levels of IL-1β at h 24 for the HBO group and the ischemia-reperfusion group were all significantly higher than that of the sham group at the same time point （P ＜0.05,P ＜0.01）.The level of IL-1β in the lung tissue of mice for the HBO group was significantly lower than that of the IR group at the same time point （P ＜ 0.05）.The level of IL-10 in the lung tissue of the HBO group was significantly higher than those of the IR group and the sham group （P ＜ 0.05）.Conclusions HBO could decrease the level of IL-1β and increase the level of IL-10 in the lung tissue of mice following cerebral ischemia-reperfusion,then it could offer some protection for acute lung injury induced by cerebral ischemia-reperfusion.

关 键 词：高压氧 脑缺血再灌注 急性肺损伤 炎性细胞因子 小鼠 

分 类 号：R563.8[医药卫生—呼吸系统]