不同产地及不同药用部位大叶蛇葡萄中蛇葡萄素和杨梅苷的含量测定  

Determination of Ampelopsin and Myricitrin in Ampelopsis megalophylla from Different Places and Medicinal Parts by RP-HPLC

作　　者：陈敏[1] 凃媛 桂春[1] 程佩佩[1] 严瀛灏 张秀桥[1] 

机构地区：[1]湖北中医药大学药学院,湖北武汉430065

出　　处：《中国现代中药》2014年第4期283-286,共4页Modern Chinese Medicine

基　　金：国家自然科学基金项目(31170335);武汉市重点科技攻关计划项目(201160823265)

摘　　要：目的:建立RP-HPLC同时测定不同产地、不同药用部位大叶蛇葡萄中蛇葡萄素和杨梅苷含量的方法。方法:大叶蛇葡萄样品先用石油醚超声脱脂,再用甲醇超声提取;分析柱选用Angilent TC-C18(250 mm×4.6 mm,5μm)色谱柱,以乙腈-0.1%磷酸水溶液进行洗脱(20∶80,v/v),柱温25℃,流速1.0 mL·min-1,检测波长254nm。结果:蛇葡萄素在16.6~415.0μg·mL-1与峰面积呈现良好的线性关系(r=0.999 8),平均回收率为98.25%,RSD=1.14%(n=6);杨梅苷在1.0~25.5μg·mL-1与峰面积呈现良好的线性关系(r=0.999 5),平均回收率为98.19%,RSD=1.16%(n=6)。结论:蛇葡萄素和杨梅苷成分因产地和药用部位不同,含量差异较大。该方法操作简便、可靠,可为大叶蛇葡萄的质量控制和开发利用提供实验依据。Objective： Establish RP-HPLC method for determining of Ampelopsin and Myricitrin in Ampelopsis megalophylla from different places and medicinal parts. Methods： After ultrasonic treatment with petroleum ether and methanol, the separation was achieved on a Angilent TC-Cls （250 mm x 4. 6 ram, 5 μm）column at 25 ℃ using methyl cyanide-0. 1% phosphoric acid solution（ 20：80, v/v）as the mobile phase at a flow rate of 1.0 mL. rain-1. The detection wavelength was 254 nm. Results： Ampelopsin showed good linear at the range of 16.6 ~415.0 i^g＇mL-1 （r =0. 999 8）. The recovery was 98.25% and RSD was 1.14%（n=6）. Myricitrin showed good linear at the range of 1.0 -25.5 p,g＇mL-1 （r =0. 999 5）. The recovery was 98. 19% and RSD was 1.16% （n = 6）. Conclusion： The contents of the two components are obviously different because from different places and medicinal parts. The proposed method is simple and reliable. It can provide experimental evidence for quality control, development and utilization of Ampelopsis megalophylla.

关 键 词：大叶蛇葡萄 蛇葡萄素 杨梅苷 

分 类 号：R284.1[医药卫生—中药学]