清热益气通络方对DN大鼠肾小球病理及氧化应激的影响  

Qingre Yiqi Tongluo Fang on Oxidative Stress and Glomerular Morphologic in Diabetic Nephropathy Rats

作　　者：朱海慧[1,2] 叶学锋[3] 谢春光[3] 

机构地区：[1]成都中医药大学临床医学院,成都610072 [2]陕西中医学院 [3]成都中医药大学附属医院

出　　处：《浙江中医药大学学报》2014年第2期196-200,共5页Journal of Zhejiang Chinese Medical University

基　　金：国家中医药管理局中医临床重点学科内分泌专项(CTX2010003-05)~~

摘　　要：[目的]研究清热益气通络方治疗大鼠糖尿病肾病的作用机制。[方法]采用单侧肾切除腹腔注射链脲佐菌素方法建立大鼠糖尿病肾病模型。将成模大鼠分为模型组、中药组和厄贝沙坦组,连续治疗12W。观察各组①肾组织超氧歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)、丙二醛(MDA)含量;②肾小球横截面积(glomerular area,GA)、系膜区面积(mesangium area,MA)、MA/GA、肾小球基底膜厚度(glomerular basement membrane thickness,GBMT)、足突平均宽度(width of the podocyte foot processes,FPW)、足突融合率(foot process fusion rate,FPFR)。[结果]与正常组比,模型组大鼠肾组织MDA增高,SOD、CAT、GSH-Px降低(P<0.01);GA、MA、FPW、FPFR及GBMT增高,MA/GA降低(P<0.01或P<0.05)。清热益气通络方能降低糖尿病大鼠肾组织MDA及GA、MA、FPW、FPFR、GBMT(P<0.01或P<0.05),升高SOD、CAT水平(P<0.01或P<0.05);其提高SOD、CAT,降低MDA及PFPR的作用优于厄贝沙坦(P<0.05)。[结论]清热益气通络方对DN具有治疗和保护作用,其机制可能与提高肾组织抗氧化物质,减轻DN大鼠肾脏氧化应激损伤,从而改善DN大鼠肾脏病理有一定关系。[Objective]To investigate the mechanism of Qingre Yiqi Tongluo（QRYQTL） fang in treating the diabetic nephropathy. [Methods]60 Sprague-Dawleg rats were divided into normal control group（N）, Diabetic nephropathy model group（M） （model rats were prepared by intraperitoneal injection with STZ after unilateral nephrectomy）, then the model rats were assigned into model control group, QRYQTL fang treatment group（Q） and Irbesartan treatment group（I）. After 12 weeks of the treatment, the contents of superoxide dismutase（SOD）,catalase（CAT）,glutathione peroxidase（GSH-Px） and malondialdehyde （MDA） in renal tissue were examined by enzyme linked immunoassay（ELISA）; the glomerular area（GA）, mesangium area（MA）,MA/GA, mean width of the podocyte foot processes（FPW） ,foot process fusion rate（FPFR） and average thickness of glomerular basement membrane（GBMT） were measured. [Results]Compared with N group, the contents of MDA in kidney homogenate and the GA,MA, FPW,FPFR,GBMT were markedly increased （P〈0.01or P〈0.05）,the contents of SOD,CAT, GSH-Px and MA/GA were significantly decreased（ P〈0.01or P〈0.05）. QRYQTL fang reduced the level of MDA and decreased the GA, FPW, FPFR and GBMT（P〈0.01or P〈0.05）. Compared with I group ,the QRYQTL fang was superior to Irbesartan in reducing the MDA, FPFR as wel as increasing the SOD,CAT（P〈0.05）.[ Conclusions]QRYQTL fang has a protective effect on the experimental diabec-tic nephropathy rats. The mechanism may be related to inhibiting oxidative stress, improving the body ’s antioxidant ability and al eviating the glomerular morphologic injury.

关 键 词：糖尿病肾病 肾小球 病理形态 氧化应激 

分 类 号：R331[医药卫生—人体生理学]