灯盏花素对糖尿病大鼠心肌及神经肽Y水平的影响  

Effect of breiscapine on myocardial intervention and neuropeptide Y levels in diabetic rats

作　　者：赵凌云[1,2] 李学英[1] 罗蔓莉[1] 许永杰[1] 刘晓红[3] 

机构地区：[1]遵义医学院细胞生物学与遗传学教研室,贵州遵义563003 [2]开封市第二人民医院肿瘤科,河南开封475000 [3]遵义医学院生理学教研室,贵州遵义563003

出　　处：《南京医科大学学报（自然科学版）》2014年第2期164-167,共4页Journal of Nanjing Medical University(Natural Sciences)

基　　金：贵州省教育厅基金资助(2004213);贵州省卫生厅基金资助(2004)

摘　　要：目的:探讨灯盏花素对糖尿病大鼠心肌的干预作用及对神经肽Y(NPY)水平的影响。方法:建立T1DM大鼠模型,将成模后的成年Wister大鼠随机分为糖尿病模型组、胰岛素治疗组、灯盏花素低剂量干预组、灯盏花素高剂量干预组,另设正常对照组。观察血糖、体重、心脏重量指数、心系数,放免法测定NPY水平,常规HE染色观察心肌组织的形态学改变。结果:糖尿病大鼠心脏重量指数、心系数以及血浆、心肌组织内NPY水平显著升高,HE染色显示心肌细胞存在等多种病理改变。胰岛素治疗与灯盏花素干预后均能改善糖尿病大鼠心肌组织的病理变化,其中灯盏花素高剂量干预效果同胰岛素治疗组。在干预和治疗过程中血浆NPY水平降低,心肌组织NPY水平升高。结论:糖尿病大鼠血浆NPY水平增高,灯盏花素可以降低血浆NPY水平,改善糖尿病大鼠心功能、心肌细胞肥大现象,并可改善糖尿病大鼠的一般生理状况。Objective：The aim of this study was to investigate the interventional effects of breviscapine on myocardial tissue of and neuropeptide Y level of diabetic rats. Methods：Experimental diabetes model of wistar rats were established by intraperitoneal injection of streptozotocin. They were randomly divided into untreated group,insulin treatment group,low dose breviscapine intervention group,high dose breviscapine intervention group and normal control group. The body weight and blood glucose at state of empty stomach were measured before execution. Furthermore,cardiac mass index and heart coefficient of rats were measured after excution. NPY levels in plasm and myocardial tissue were measured by radioimmunoassay（RIA）. The pathologic changes of myocardium were observed using HE staining. Results：Cardiac mass index,heart coefficient and NPY levels in plasma and myocardial tissue of diabetic rats were significantly increased. Various pathological changes were observed in diabetic rats. TRI and breviscapine intervention improved myocardial tissue pathological changes of diabetic rats,the effect of high dose intervention of breviscapine is similar to that of insulin therapy. NPY levels were decreased in plasma during breviscapine intervention,while increased in myocardial tissue. The results showed that NPY levels were significantly higher in diabetic rats as compared with control group. Conclusion：Breviscapine didn＇t reduce the level of blood glucose,however,it improved physiological functions,such as myocanrdium hypertrophy and cardiac function in rats with diabetic cardiomyopathy.

关 键 词：灯盏花素 糖尿病 心肌 神经肽Y 

分 类 号：R587.1[医药卫生—内分泌]