大鼠下腔静脉血栓模型的建立及手术技巧  

Rat inferior vena cava thrombosis model and surgical techniques

作　　者：白云城[1] 赵学凌[1] 周如丹[1] 周志化[1] 吴雪梅[1] 王兵[1] 

机构地区：[1]昆明医科大学第一附属医院骨科,云南昆明650032

出　　处：《南京医科大学学报（自然科学版）》2014年第3期394-399,共6页Journal of Nanjing Medical University(Natural Sciences)

基　　金：国家自然科学基金面上资助项目(81160236);云南省卫生科技项目(2011WS0034;2012WS0007);云南省博士研究生学术新人奖项目

摘　　要：目的:建立大鼠下腔静脉血栓模型,总结建立模型过程中的手术技巧与注意事项。方法:健康SD大鼠64只,分为对照组和造模组,造模组采用"狭窄法",阻断下腔静脉大部分血流,通过结扎后相应时间点开腹观察和取材,进行病理学检查,评价造模是否成功。结果:对照组和造模组在实验过程中均未出现意外死亡,生存率100%。对照组下腔静脉无血栓形成(0/8);造模组在狭窄法术后2 h可见有血栓形成(6/8,75%),至术后6 h均可见血栓形成(8/8,100%),术后24 h和48 h,血栓形成,同时管腔内明显充血(16/16,100%),术后7 d血栓有机化表现,但未出现明显消退(8/8,100%),术后14 d至术后21 d观察到血栓溶解消退(16/16,100%)。结论:采用狭窄法使下腔静脉血流淤滞,可成功建立下腔静脉血栓模型。Objective：To establish a rat model of inferior vena cava thrombosis,and to summarize the process of modeling techniques and surgical considerations. Methods：A total of 82 healthy SD rats were divided into the control group and the model group. The model group was adopted a ＂stenosis technology＂,which blocked most of blood flow in the inferior vena cava. At determined time points after ligation,laparotomy was performed and tissues were observed and sampled for pathological examination to evaluate modeling success. Results：Both of the two groups survived during the whole experiment and there were no accidental death,with the survival rate of 100%. The control group had no inferior vena cava thrombosis（0 / 8）;in the Model group,thrombosis（6 / 8,75%）was found after two hours of surgery,and it was visible after 6 hours（8 / 8,100%）. Between 24 to 48 hours after surgery,a stable thrombus formed with a lumen hyperemia（16 / 16,100%）. After 7 days,organic thrombus was observed,but there was no significant regression（8 / 8,100%）. The dissolving thrombus regression was observed between 14 to 21 days after surgery（16 / 16,100%）. Conclusion：The stenosis technology can be used to block inferior vena cava blood flow successfully to establish the inferior vena cava thrombosis model.

关 键 词：血栓 血管生物学 静脉血栓 动物模型 大鼠 

分 类 号：R619.2[医药卫生—外科学]