青岛地区2008—2011年柯萨奇病毒A组16型流行及VPl基因特征  

Coxsackievirus A16 epidemiology and VP1 gene characteristics in Qingdao region,2008 -2011

作　　者：刘晓琳[1] 王慧[1] 柴青[1] 汪照国[1] 

机构地区：[1]青岛市疾病预防控制中心卫生微生物室,山东266033

出　　处：《中华实验和临床病毒学杂志》2014年第2期123-125,共3页Chinese Journal of Experimental and Clinical Virology

基　　金：青岛市科技局项目（08-2-1-1-nsh）

摘　　要：目的阐明2008—2011年青岛地区手足口病（hand，foot，andmouthdisease，HFMD）相关柯萨奇A组16型病毒（CoxsackievirusA16，CAl6）流行情况及VPl基因特征。方法首先采用荧光RT—PCR法对HFMD患者咽拭子标本进行总肠道病毒（enterovirus，EV）、柯萨奇病毒A组16型（CAl6）和人肠道病毒71型（humanenterovirus71，EV71）检测；对CAl6阳性的标本进行病毒分离培养，选取阳性分离株的代表株进行VP1基因全长序列的扩增及基因序列测定，并与GenBank中的CAl6代表株进行核苷酸和氨基酸的同源性和亲缘性分析。结果2008—2011年青岛地区CAl6在HFMD相关肠道病毒中所占比例分别为39．O％、10．6％、46．9％和2．O％。46株CAl6病毒VPl基因核苷酸和氨基酸的差异性分别为11．0％和2．O％。所有VPl序列在进化树上可分为2个分支，分别属于B1a亚型和B1b亚型。2008—2011年间CAl6分离株中B1a亚型与B1b亚型的数量分别为9／7、7／1、3／11和2／6。结论2008—2011年CAl6青岛地区每两年出现一次流行高峰，B1a和B1b两个亚型一直共循环流行，2009年与2010年分别以B1a亚型和B1b亚型为优势流行，至2011年CAl6在HFMD的病原谱中检出率极低。Objective To illuminate the molecular epidemiology and gene characteristics of Coxsaekievirus A16 （CA16） strains isolated from patients with hand, foot, and mouth disease（HFMD） in Qingdao region from 2008 to 2011.Methods The throat swabs of cases with HFMD were detected for enterovirns （EV）, EV71 and CA16 by fluorescence reverse transcription-polymerase chain reaction （RT- PCR）. The EV positive specimens were inoculated on appropriate cultures for virus isolation. The whole VP1 gene sequences of CA16 isolates were amplified and sequenced, then the homology and phylogeny analysis were conducted with CA16 international sequences obtained from GenBank database. Results The proportion of CA16 in etiology of HFMD from 2008 to 2011 was respectively 39.0% , 10. 6% , 47.0% and 2. 0%. The nueleotide and amino acid difference of VP1 gene were respectively 11.0% and 2. 0%. The sequences of 46 CA16 isolates were classified into two subgenotypes of Bla and Blb. The number of Bla and Blb from 2008 to 2011 were respectively 9/7, 7/1, 3/11 and 2/6. Conclusion The large prevalence of CA16 occurred at intervals of about two years in Qingdao from 2008 to 2011. Bla and Blb clusters were co- circulating, especially Bla was predominant in 2009 and Blb was predominant in 2010 and till 2011 the detective rate of CA16 became extremely low in etiology of HFMD.

关 键 词：手足口病 柯萨奇病毒感染 基因 

分 类 号：R725.1[医药卫生—儿科]