慢性荨麻疹血清中组胺释放因子(HRF)及HRF反应性IgE的检测  

Detecting Histamine-Releasing Factor( HRF) and HRF-reactive IgE in the Sera of Patient with Chronic Urticaria

作　　者：李朝阳[1] 孙仁山[1] 李菁[1] 宋君[1] 伍津津[1] 杨涛[1] 唐辉[1] 唐书谦[1] 李凤[1] 杨永强[1] 

机构地区：[1]第三军医大学大坪医院野战外科研究所皮肤科,重庆400042

出　　处：《中国皮肤性病学杂志》2014年第5期461-463,共3页The Chinese Journal of Dermatovenereology

基　　金：国家自然科学基金(30972647)

摘　　要：目的研究组胺释放因子(histamine-releasing factor,HRF)及HRF反应性IgE在慢性荨麻疹患者发病中的作用。方法对40例慢性荨麻疹患者进行活动性评分(the urticaria activity score,UAS),分为重症组和轻症组,采用Elisa酶联免疫吸附试验检测荨麻疹患者发病时血清中HRF及HRF反应性IgE含量,并与40例正常人的检测结果作对照。结果患者组和正常对照组血清中HRF平均值分别为8.81ng/mL和4.30ng/mL,差异有统计学意义(P<0.05)。轻症组和重症组的血清HRF平均值分别为4.65 ng/mL和11.06ng/mL,差异有统计学意义(P<0.05)。患者组和正常对照组血清HRF反应性IgE抗体平均值分别为1.67IU/mL和0.55IU/mL,差异有统计学意义(P<0.05),重症组和轻症组均数分别为1.30IU/mL和0.91IU/mL,差异有统计学意义(P<0.05)。直线相关性分析结果显示HRF及同一患者HRF反应性IgE抗体的含量,存在明显正相关性(P=0.006)。结论慢性荨麻疹患者发病时血清中HRF含量及HRF反应性IgE与病情有着显著正相关,说明HRF及HRF反应性IgE在慢性荨麻疹发病中发挥重要作用。Objective To explore the significances of Histamine-Releasing Factor （HRF）and HRF-reactive IgE in the pathogenesis of chronic urticaria（CU）. Methods The serum concentration of HRF and HRF-reactive IgE in 40 chronic urticaria patients were tested by enzyme-linked immunosorbent assay（ELISA）. Forty healthy vol- unteers were selected as the control. Results The average serum concentration of HRF was 8.81 ng/mL in patient group;4. 30ng/mL in control group;4. 65 ng/mL in mild group and 11.06ng/mL in severe group. The serum level of HRF in patient group differed significantly from control group （P 〈 0. 05）. The average serum concentration of HRF in severe group was significantly higher than the mild group （ P 〈 0. 05 ）. The average serum concentration of HRF-reactive IgE in patient group was also significantly higher than control group （ 1.67IU/mL vs. 0. 55IU/mL） （P 〈 O. 05 ）. The average serum concentrations of HRF-reactive IgE in mild group and severe group were 0. 91IU/mL and 1.30IU/mL respectively. It proved a significant differ- ence between mild group and severe group （P 〈 0.05 ）. The significant linear correlation between HRF and HRF-reactive IgE was found（P =0. 006）. Conclusion The levels of HRF and HRF-reactive IgE in serum both correlated positively with chronic urticaria activity score, which confirmed that HRF and HRF-reactive IgE may take very important roles in the pathogenesis of chronic urticaria（CU）.

关 键 词：荨麻疹 慢性 组胺释放因子 组胺释放因子反应性IgE 荨麻疹活动性评分 

分 类 号：R758.24[医药卫生—皮肤病学与性病学]