透明颤菌血红蛋白基因克隆及植物表达载体的构建  

Cloning of VHb Gene and Construction of Plant Expression Vector

作　　者：肖宇[1] 孙力[1] 王晓飞[1] 高宇[1] 

机构地区：[1]黑龙江省科学院大庆分院区域资源与环境研究所,黑龙江大庆163319

出　　处：《生物技术》2014年第2期41-44,共4页Biotechnology

基　　金：黑龙江省科学院青年创新基金项目("转VHb基因沉水植物改善水体污染的研究与分析";编号:CX12G02)资助

摘　　要：透明颤菌血红蛋白是一种专性好氧的氧结合蛋白。目的:以VHb基因构建植物表达载体,并在大肠杆菌中实现表达。方法:使利用试剂盒提取透明颤菌血红蛋白的总DNA,以其为模板通过PCR克隆VHb基因。结果:克隆得到VHb基因片段的长度约为0.5kb,与NCBI上公布的VHb基因序列最大同源性达99%。结论:通过酶切鉴定及PCR检测,成功构建重组植物表达载体p3300-vhb-super promoter-Tnos。bstract ： Vitreoscilla hemoglobin is an oxygen regulated, oxygen binding protein. Objective： Plant expression vector was constructed by u- sing the gene and expression in Escherichia coll. Method： The total DNA was extracted by Kit Vitreoscilla hemoglobin, and VHb gene was cloned by PCR cloning from this DNA template. Result： The length of VHb gene sequence is about 0. 5 kb. The maximum homology was 99% compared with opened NCBI sites. Conclusion： By enzyme digestion and PCR detection, recombinant plant expression vector p3300 - vhb - super promoter -Tnos was constructed successfully.

关 键 词：透明颤菌血红蛋白 VHB基因 植物表达载体 水花生 

分 类 号：Q785[生物学—分子生物学]