高生物量草分枝杆菌突变株诱变选育的研究  

Study on Mutation Breeding of Mycobacterium phlei with High Living Weight

作　　者：祝芳[1] 彭剑勇[1] 于晓丹[1] 王铜生 李楠[1] 吕淑霞[1] 马镝[1] 

机构地区：[1]沈阳农业大学生物科学技术学院,辽宁沈阳110866

出　　处：《生物技术》2014年第2期76-80,共5页Biotechnology

基　　金：国家青年科学基金项目("香蘑属系统发育关系的重建与DNA条形码的筛选";No.31200011);沈阳农业大学青年基金项目(No.20111012)资助

摘　　要：目的:为了获得具有提高人体免疫力的草分支杆菌菌株的生长量。方法:采用紫外线和硫酸二乙酯两种方法,对其原始菌株进行复合诱变,筛选生长能力强的突变菌株。结果:实验结果表明紫外线诱变最佳处理时间为90 s,硫酸二乙酯诱变最佳处理时间为50 min,筛选出生长能力强的3株突变株(50min-1、50min-4和50min-7),其生长能力较原菌株分别提高了62%、57%和59%。通过方差分析来检测这3株突变菌株的遗传稳定性,结果显示传代次数对突变株50min-1和50min-4的生长量影响不显著,而对50min-7的生长量的影响显著,因此50min-1和50min-4具有遗传稳定性。结论:研究结果为草分支杆菌的发酵研究提供了优良菌株。Objective： In order to obtain the mutant strains of Mycobacterium phlei with high living weight. Method： Based on both ultra vi- olet and diethyl sulfate, the mutant strains with high living weight were selected. Result：The results showed that the optimal treatment time of ultra violet is 90 s and the best treatment time of DES is 50 min. There were three mutant strains of Mycobacterium phlei （50min - 1, 50min - 4 and 50min - 7 ）, the growth ability increased by 62 % , 57 % and 59 % , respectively. Finally, the results of variance analyses of generation test of mutant strains showed that 90s - 1 has the genetic stability, while the hereditary of other two strains are not stability. Conclusion： The results of this study provide the good strains for the fermentation research of Mycobacterium phlei.

关 键 词：分支杆菌属 紫外线 硫酸二乙酯 物理诱变 化学诱变 致死率 遗传稳定性 

分 类 号：Q815[生物学—生物工程]