2株狼尾草根际芽孢杆菌(Bacillus spp.)的分离与特性分析  

Isolation and Characterization of Two Bacillus spp. from the Rhizosphere of Pennisetum alopecuroides

作　　者：王晓丹[1] 刘佳莉[1] 张丹[1] 郭长虹[1] 

机构地区：[1]哈尔滨师范大学生命科学与技术学院,分子细胞遗传与遗传育种黑龙江省重点实验室,黑龙江哈尔滨150025

出　　处：《生物技术》2014年第2期95-98,共4页Biotechnology

基　　金：国家自然科学基金项目("不同修复植物对石油污染研究土壤根际微生物群落的影响研究";No.31170479);黑龙江省科技攻关项目("苜蓿根际促生菌的开发与利用研究";GC12B304);黑龙江省自然科学基金项目("石油污染盐碱土壤植物促生菌的筛选及利用研究";C201142)资助~~

摘　　要：目的:从狼尾草根际土中分离出具有多种生物学特性和促生效果的芽孢杆菌。方法:土样经过80℃处理,得到2株菌。对其溶磷、合成IAA和嗜铁素能力进行测定。用2株菌处理小麦种子,评价其促生效果。结果:菌株WXD 2-1和WXD 2-2溶解磷的能力分别为(26.99±1.74)μg/mL和(31.99±4.43)μg/mL;随着L-Trp浓度的增加,两个菌株的IAA合成量也相应增加;菌株WXD 2-2合成嗜铁素能力高于菌株WXD 2-1。分别用菌株WXD 2-1和WXD 2-2处理小麦种子,生物量与对照相比均有显著增加(p<0.05),且菌株WXD 2-1促生效果较菌株WXD 2-2更好。结论:分离出的芽孢杆菌具有溶磷、合成IAA和嗜铁素能力,能够促进小麦的生长。To isolating the Bacillus strains from the rhizosphere of Pennisetum alopecuroides, which have a variety o~ biological characteris- tics and growth promotion ability. Method： In the study, using high - temperature method to isolate Bacillus spp.. Planted the wheat seeds soaked with the suspension liquid of strains, and evaluate the effect of the growth - promoting after 30. days. Result： The phosphate solubilization of WXD 2 - 1 and WXD 2 - 2 were （26. 99± 1.74） g/mL and （31.99 ± 4. 43 ） μg/mL, respectively. With the concentra- tion of L - Trp increased, the IAA syntheses by two strains were increased. The ability of synthesize siderophore of WXD 2 - 2 was higher than WXD 2 - 1. The biomass of wheat treated with WXD 2 - 1 and WXD 2 - 2 were increased significantly（p 〈 0. 05 ）. Conclusion： Two strains have phosphate solubilization, IAA syntheses, siderophore syntheses, and growth promotion ability.

关 键 词：芽孢杆菌 嗜铁素 溶磷 吲哚乙酸 促生 

分 类 号：Q939.124[生物学—微生物学]