DETA/NO降低大鼠蛛网膜下腔出血后微血管痉挛及早期脑损伤作用  

Effects of DETA/NO on capillary spasm and early brain injury after subarachnoid hemorrhage in rats

作　　者：李强[1,2] 冯华[2] 喻安永[2] 陈景宇[2] 赵喆[1] 吴国材[2] 阮怀珍[1] 

机构地区：[1]第三军医大学基础部神经生物学教研室,重庆400038 [2]第三军医大学西南医院神经外科,重庆400038

出　　处：《西安交通大学学报（医学版）》2014年第3期352-356,360,共6页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：国家自然科学基金重大国际合作项目(No.81220108009);国家自然科学基金面上项目(No.31171069)~~

摘　　要：目的通过二乙烯三胺/一氧化氮聚合物(DETA/NO)对雄性SD大鼠线穿法建造的蛛网膜下腔出血(SAH)模型的干预,观察其对微血管痉挛的防治及降低早期脑损伤作用。方法将69只雄性SD大鼠随机分为假手术组(sham组)、对照组(SAH组)和治疗组(DETA/NO组)。通过线穿建立SAH模型,观察大体动物Garcia神经功能评分,免疫荧光染色检测微血管肌动蛋白αSMA及PDGFRβ的表达,一氧化氮(NO)试剂盒检测脑组织NO浓度变化,观察血红蛋白刺激离体脑片微血管变化情况。结果手术后3d,DETA/NO组较SAH组神经功能评分明显改善(P<0.05);免疫荧光结果显示,SAH后大脑皮层微血管周围αSMA、PDGFRβ表达上调(P<0.05),DETA/NO能在一定程度上逆转此过程(P<0.05);术后SAH 3h左右NO浓度显著降低(P<0.05),此后逐渐回升,DETA/NO能在早期维持NO浓度(P<0.05);观察到脑片皮层微血管受血红蛋白刺激收缩,DETA/NO能缓解微血管痉挛。结论 DETA/NO能降低大鼠蛛网膜下腔出血后微血管痉挛及早期脑损伤。Objective To explore the effects of diethylenetriamine/nitric oxide （DETA/NO） on capillary spasm and early brain injury （EBI） after subarachnoid hemorrhage （SAH） in rats. Methods Sixty-nine male Sprague-dawley rats were randomized into three groups： sham group, SAH group and DETA/NO group. SAH model was established by wearing out the willis ring with thread and then Garcia neurological score was observed in the general animals. The expressions of alpha smooth muscle actin （c^SMA） and PDGFR^3 were detected by dual immunofluoreseence staining; nitric oxide kit was used for detecting brain tissue NO concentration. Changes in the hemoglobin-stimulated capillaries were observed in rat slices. Results Three days after surgery, neurological deficit score was remarkably improved in DETA/NO group compared with that in SAH group （ P ~ 0. 05）. Immunofluorescence results showed that the expressions of peri-capillary aSMA and PDGFR/3 were significantly increased after SAH （P ~ 0. 05）, and that DETA/NO could down-regulate the expressions （P ~ 0. 05）. NO concentration was greatly reduced about 3 hours after SAH and then gradually increased; DETA/NO could maintain the concentration of NO at an early stage （P^0.05）. The capillary contraction was observed in slices perfused with hemoglobin; DETA/NO could alleviate capillary spasm. Conclusion capillary spasm and EBI after SAH in rats. DETA/NO can alleviate the severity of

关 键 词：蛛网膜下腔出血 早期脑损伤 微血管痉挛 二乙烯三胺 一氧化氮聚合物(DETA NO) 一氧化氮 

分 类 号：R331.3[医药卫生—人体生理学]