8-溴-7-甲氧基白杨素逆转人宫颈癌SiHa细胞系球形成细胞上皮-间叶样表型转化  

8-bromo-7-methoxychrysin reverses EMT of the sphere-forming cells derived from cervical cancer SiHa cell line

作　　者：曹晓正 杨小红[1] 曹建国[1] 向红琳[1] 

机构地区：[1]湖南师范大学医学院药物工程实验室,湖南长沙410013

出　　处：《中国药理学通报》2014年第5期668-671,共4页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No 81172375)

摘　　要：目的研究8-溴-7-甲氧基白杨素(8-bromo-7-methoxychrysin,BrMC)能否有效逆转人宫颈癌SiHa细胞系球形成细胞上皮-间叶样表型转化(epithelial-mesenchymal transition,EMT)。方法体外培养SiHa细胞系细胞,干细胞条件培养基悬浮培养得到球形成细胞(sphere forming cells,SFCs)。以BrMC处理SFCs后,通过单层贴壁培养观察细胞形态。Western blot分析SFCs的E-cadherin、N-cadherin和Twist1蛋白表达水平。结果与亲本细胞比较,SiHa细胞系SFCs呈现间叶样细胞(梭形细胞)形态,并低表达E-cadherin和高表达N-cadherin蛋白。BrMC(1.0μmol·L-1)处理72 h后,SFCs由梭形细胞转变为多边形细胞;BrMC(0.1和1.0μmol·L-1)作用24 h,SFCs中E-cadherin表达上调,N-cadherin和Twist1蛋白表达降低。结论 BrMC具有逆转SiHa细胞系SFCs的EMT作用,其机制涉及降低Twsit1蛋白表达。Aim To investigate whether 8-bromo-7- methoxychrysin （BrMC） can reverse the epithelialmesenchymal transition （EMT） in sphere-forming cells （SFCs） derived from cervical cancer SiHa cell line. Methods Human cervical cancer SiHa cell line was cultured in vitro and then SFCs were obtained from SiHa cell line using suspension culture with the stem- condition culture system. After treatment with BrMC for 72 h, the morphology of SFCs of SiHa cell line was observed via monolayer adherence culture. The expression levels of E-cadherin, N-eadherin and Twistl proteins were analyzed using western blot assay. Results SFCs of SiHa cell line like （spindle-shaped） cell E-eadherin and elevated presented the mesenchymeshape and exhibited reduced N-cadherin protein expres-sion, compared with the parental cells. After treatment with BrMC （ 1.0 umol . L-1 ） for 72 h, SFCs of SiHa cell line changed from spindle-shaped cells in to polygon shaped ceils under monolayer adherence culture. After treatment with BrMC for 24 h, the expression lev-els of E-cadherin protein were up-regulated and the expression levels of N-cadherin and Twistl proteins were decreased in SFCs derived from SiHa cell line. Con-clusion BrMC effectively reverses EMT of SFCs derived from cervical cancer SiHa cell line, and its mechanism is involved in down-regulating the expression of Twist protein.

关 键 词：肿瘤干细胞 宫颈癌 球形成细胞 8-溴-7-甲氧基白 杨素 上皮-间叶样表型转化 TWIST1 

分 类 号：R284.1[医药卫生—中药学] R329.24[医药卫生—中医学]