二甲双胍对大鼠肾小球系膜细胞NF-κB、MCP-1、ICAM-1表达及合成的影响  

Effects of metformin on expression and synthesis of intercellular adhesion molecule-1,monocyte chemoattractant protein-1 and nuclear factor-κB in rat glomerular mesangial cells

作　　者：顾俊菲[1] 叶山东[1] 汪姗[1] 孙文佳[1] 胡圆圆[1] 

机构地区：[1]安徽医科大学附属省立医院内分泌科,合肥230001

出　　处：《安徽医科大学学报》2014年第5期582-585,共4页Acta Universitatis Medicinalis Anhui

基　　金：安徽省自然科学基金(编号:11040606M161);安徽高校省级自然科学研究项目(编号:KJ2011A157)

摘　　要：目的观察二甲双胍对大鼠肾小球系膜细胞(MCs)核因子-κB(NF-κB)、单核细胞趋化蛋白-1(MCP-1)、细胞间黏附分子-1(ICAM-1)表达及合成的影响,探讨其对肾脏的保护机制。方法体外培养MCs,随机分为正常糖组(NG)、高糖组(HG)及高糖+不同浓度二甲双胍组(M1、M2、M3)。培养48 h后收集各组细胞及上清液,采用荧光实时定量聚合酶链式反应(Real-time PCR)法检测细胞NF-κB、MCP-1及ICAM-1的mRNA含量;采用Western blot法检测细胞NF-κBp65及ICAM-1蛋白表达水平;采用ELISA法检测上清液中MCP-1蛋白浓度。结果①MCs可表达NF-κB、MCP-1和ICAM-1;②与NG组比较,HG组MCs NF-κB、MCP-1、ICAM-1的mRNA表达增强,NF-κBp65、ICAM-1及细胞上清液中MCP-1蛋白含量增高(P<0.05);③经二甲双胍干预后,高糖培养的MCs NF-κB、MCP-1及ICAM-1表达合成显著降低(P<0.05)。结论二甲双胍可抑制MCs NF-κB、MCP-1和ICAM-1的表达及合成,该作用可能与其肾脏保护机制有关。Objective To observe the effects of metformin on expression and synthesis of nuclear factor-κB （ NF-κB）, monocyte chemoattractant protein-1（MCP-1） and intercellular adhesion molecule-1（ICAM-1） in cultured rat glomerular mesangial cells（ MCs） and explore its reno-protective mechanisms. Methods MCs were cultured in the medium with normal glucose （ group NG ） , high glucose （ group HG ） and different concentrations of metformin （group M1, M2, M3）. After 48 h exposure, the supernatants and MCs were collected. The expression of NF-κB, MCP-1 and ICAM-1 mRNA was analyzed by Real-time PCR and MCP-1 protein synthesis by ELISA. NF-κBp65 and ICAM-1 were visualized by Western blot. Results ①MCs could express NF-κB, ICAM-1and MCP-1.②Af-ter being stimulated by high glucose, the levels of intracelluar NF-κB, MCP-1 and ICAM-1 were significantly in-creased compared with group NG（P〈0.05）. ③The levels of NF-κB, MCP-1 and ICAM-1 were significantly de-creased in group M1, M2 and group M3 compared with group HG（P〈0.05）. Conclusion Metformin can sup-press the expression and synthesis of NF-κB, MCP-1 and ICAM-1 of glomerular mesangial cells, which may partly contribute to its reno-protection.

关 键 词：二甲双胍 核因子-KB 单核细胞趋化蛋白-1 细胞间黏附分子-1 

分 类 号：R349.51[医药卫生—基础医学] R587.24