DNMT3A和Hyp在ISO诱导大鼠心肌纤维化中的表达及相关性研究  

Expression and correlation of DNMT3A and Hyp of isoprenaline induced myocardial fibrosis in rats

作　　者：汪裕琪 石开虎[1,2] 吴君旭[1,2] 徐盛松[1,2] 陶辉[1,2] 宣海洋[1,2] 曹炜[1,2] 沙纪名[1,2] 占红英[1,2] 

机构地区：[1]安徽医科大学第二附属医院心胸外科,合肥230601 [2]安徽医科大学心血管病研究中心,合肥230032

出　　处：《安徽医科大学学报》2014年第5期606-609,共4页Acta Universitatis Medicinalis Anhui

基　　金：安徽省自然科学基金项目(编号:1308085MH117);安徽省高等学样省级自然科学研究重点项目(编号:KJ2011A175)

摘　　要：目的探讨DNA甲基转移酶3A(DNMT3A)和羟脯氨酸(Hyp)在盐酸异丙肾上腺素(ISO)诱导的大鼠心肌纤维化中的表达及相关性研究。方法将40只雄性SD大鼠随机等分为正常对照组和模型组。模型组给予ISO[5 mg/kg·d)],正常对照组皮下注射给予等量生理盐水,连续7 d后处死大鼠获取血液标本,并取心肌组织。同时,测定心重指数(HW/BW)、左心室质量指数(LVW/BW);采用ELISA法检测血清中Ⅰ型胶原和Ⅲ型胶原的含量;HE染色和Masson染色法观察心肌纤维化程度;Western blot法测定DNMT3A和α平滑肌肌动蛋白(α-SMA)的表达;紫外分光光度法测定心肌组织中Hyp的含量。结果与正常对照组比较,模型组HW/BW、LVW/BW明显增加;模型组血清标本中Ⅰ型胶原和Ⅲ型胶原的含量较正常对照组明显增加;HE染色和Masson染色显示模型组心肌组织出现明显的胶原纤维增生;Western blot法检测显示模型组DNMT3A和α-SMA的表达明显高于正常对照组(P<0.05,P<0.01);同时,紫外分光光度法检测显示模型组心肌组织中Hyp含量明显增高(P<0.05);DNMT3A和Hyp在大鼠心肌纤维化组织中的表达呈正相关(r=0.675,P<0.05)。结论 DNMT3A表达上调在心肌纤维化的形成过程中起重要促进作用,可能与Hyp升高有一定的相关性。Objective To investigate the expression and correlation of DNA methyltransferase 3A（ DNMT3A） and Hyp of isoprenaline（ ISO） induced myocardial fibrosis in rats. Methods 40 male SD rats were randomly divided into normal control group and model group. The model group was treated subcutaneously with 5 mg/（ kg＆#183;d） iso-prenaline daily for 7 days, but the normal control group was given the same amount of normal saline at the same time. Rats were executed to obtain blood samples and myocardial tissue. And HW/BW, LVW/BW were tested. ELISA was used to detect the content of type Ⅰ collagen and type Ⅲ collagen in serum. The degree of myocardial fibrosis was observed with HE staining and Masson staining method. The expression of DNMT3A and α-SMA was tested by Western blot. The content of hydroxproline in myocardial tissue was measured by UV spectrophotometry. Results HW/BW and LVW/BW increased significantly in the model group compared with the normal control group. The content of type Ⅰ collagen and type Ⅲ collagen in serum increased significantly in the model group compared with the normal control group. HE and Masson staining showed myocardial tissue in model group ap-peared obviously collagen fiber hyperplasia. The expression of DNMT3A andα-SMA in the model group was higher than the normal control group. UV spectrophotometric detection showed the content of Hyp in myocardial tissue in the model group increased obviously. The expression of DNMT3A andα-SMA in myocardial fibrosis tissue was pos-itively correlated（r=0.675, P〈0.05）. Conclusion DNMT3A upregulation plays an important role in the myo-cardial fibrosis, and Hyp may increase a certain correlation.

关 键 词：心肌纤维化 DNMT3A HYP 胶原 

分 类 号：R542.23[医药卫生—心血管疾病]