低压缺氧预处理对暴露于+Gz加速度所致大鼠脑组织损伤的保护作用  

Protective Action of Hypobaric Hypoxia Preconditioning on Brain Injuries in Rats After + Gz Exposure

作　　者：叶博[1] 张国荣[1] 尹哲[1] 陶磊[1] 黄俊梅[1] 

机构地区：[1]空军总医院麻醉科,北京100142

出　　处：《航天医学与医学工程》2014年第2期149-151,共3页Space Medicine & Medical Engineering

基　　金：全军"十二五"课题(CKJ11J021)

摘　　要：目的研究低压缺氧预处理对加速度环境下脑细胞病理生理变化的影响,探讨低压缺氧预处理的保护机制。方法 24只雄性SD大鼠随机分为3组(n=8),Con组为空白对照组,HHP+10 Gz组为低压缺氧预处理后暴露10 Gz加速度组,10 Gz组为直接暴露10 Gz加速度组,应用Morris水迷宫检测各组大鼠学习记忆能力,取大鼠脑组织检测SOD、CAT、GSH-PX、GSH、MDA和HSP-70含量。结果登台潜伏期:Con组大鼠全部登台,登台潜伏期为9.828±1.291s;HHP+10 Gz组有2只大鼠登台,登台潜伏期分别为31.45s和42.78s;10 Gz组仅有1只大鼠成功登台,登台潜伏期为92 s;SOD、GSH-PX、HSP-70:Con组大于10 Gz组,HHP+10 Gz组大于10 Gz组;CAT含量各组间差异无统计学意义;GSH:Con组大于HHP+10 Gz组,Con组大于10 Gz组,HHP+10 Gz组大于10 Gz组;MDA:Con组小于HHP+10 Gz组,Con组小于10 Gz组。结论低压缺氧预处理可改善加速度对大鼠学习记忆能力的损害,降低脑组织氧化损伤,其机制与增强大鼠体内抗氧化酶活性有关。Objective To evaluate the effect of hypobaric hypoxia preconditing （HHP） on ceretbral cell pathological physiology changes under acceleration environment, exploring the protection mechanism of HHP. Methods Twenty-four male SD rats were randomly divided into 3 groups （n = 8 ） , group Con was the blank control group, group HHP ＋ 10 Gz was hypobaric hypoxic preconditioning then exposedure to 10 Gz accelera- tion group, group 10 Gz was directly exposed to 10 Gz acceleration. All rats of 3 groups were tested the learn- ing and memory ability by Morris water maze test, while SOD, CAT, GSH-PX, GSH, MDA and HSP-70 con- tents of the brain tissue of rats were determined. Results Escape latencies to the plate ： all 6 rats got on the plate m group C , the latency average was （P. 525 ± 1.291）s;there were two rats to get on me plate m group HHP ＋ 10 Gz, the lantencies were 31.45 s and 42.78 s respectively; there was only one rat to get on the plate in group l0 Gz,the latency was92 s; SOD , GSH-PX , HSP-70：group Con〉group l0 Gz, group HHP ＋ 10 Gz 〉 groupl0 Gz;CAT：there was no difference among the 3 groups; GSH： group Con 〉 group HHP ＋ 10 Gz; and group C 〉 group 10 Gz;group HHP ＋ 10 Gz 〉 10 Gz;MDA：group C 〈 group HHP ＋ l0 Gz, and group C 〈 group 10 Gz. Conclusion Hypobaric hypoxia preconditioning can decrease the damage on the learning and memory ability of rats by acceleration, mechanism is related to enhancing the reduce oxidative damage of brain tissue activity of antioxidant enzymes in rats. caused by acceleration, and the mechanism is related to enhancing the activity of antioxidant enzymes in rats.

关 键 词：+Gz加速度应激 低压缺氧预处理 脑损伤 抗氧化 

分 类 号：R852.21[医药卫生—航空、航天与航海医学]