青花菜谷胱甘肽-S-转移酶基因克隆及其表达分析  

Cloning of GSTand Its Expression in Broccoli(Brassica oleracea var.italica)

作　　者：高世超[1] 林义章[1] 钟凤林[1] 赵瑞丽[1] 林琳琳[1] 占丽英 

机构地区：[1]福建农林大学园艺学院,福州350002

出　　处：《西北植物学报》2014年第4期651-657,共7页Acta Botanica Boreali-Occidentalia Sinica

基　　金：福建省大宗蔬菜产业体系(2012K83139294);福建省自然科学基金(2012J01082);福建农林大学校重点建设项目(6112c0409)

摘　　要：为探讨青花菜在模拟酸雨胁迫下谷胱甘肽-S-转移酶的表达变化,克隆了青花菜谷胱甘肽-S-转移酶基因(glutathione-S-transferase,GST)的cDNA序列全长,并进行了生物信息学和表达分析。结果表明:青花菜GST基因cDNA全长为915bp,开放阅读框为642bp,编码213个氨基酸,推测分子式为C1091H1719N289O306S5,分子量为23 940.7,没有跨膜螺旋区域和信号肽。系统进化树分析表明,该青花菜基因GST与芥菜的GST聚类关系最近。实时荧光定量PCR结果显示,在模拟酸雨胁迫下,GST基因的表达量在胁迫初期显著增大,随时间延长开始下降,表明其参与了青花菜抗酸雨的应答反应。The full-length cDNA of GST was cloned in order to explore molecular response mechanism in broccoli under acid rain stress, which was analyzed by bioinformatics method and real-time PCR. The re- sults showed that the full-length DNA was of 915 bp,opening reading frame（ORF） was of 642 bp. And the ORF encoded 213 amino acids,suggesting a formula of C1091 H1719 N289 O306 S5 ,a molecular weight of 23 940.7,had no transmembrane helix region or signal peptide. Phylogenetic tree analysis revealed that this gene of broc- coli had closer relationship with that from Brassica juncea. The analysis by real-time PCR suggested that the expression of GST genes in broccoli under acid rain stress increased significantly at first,and then de- clined due to longer duration of acid rain stress, which was involved in signaling pathways in response to the acid rain stress.

关 键 词：青花菜 谷胱甘肽-S-转移酶基因 分子克隆 模拟酸雨 表达 

分 类 号：Q785[生物学—分子生物学] Q786