甘蔗条纹花叶病毒 P3 蛋白与甘蔗 Rubisco 大亚基互作的研究  

Interaction between Sugarcane Streak Mosaic Virus P3 and Rubisco Large Subunit from Sugarcane

作　　者：朱海龙[1] 程光远[1] 彭磊[1] 柴哲[1] 郭晋隆[1] 许莉萍[1] 徐景升[1] 

机构地区：[1]福建农林大学农业部福建甘蔗生物学与遗传改良重点实验室,福州350002

出　　处：《西北植物学报》2014年第4期676-681,共6页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家863课题(2013AA102604);国家自然科学基金(31171605,31371688)

摘　　要：该研究以甘蔗条纹花叶病毒(Sugarcane streak mosaic virus,SCSMV)的P3蛋白(SCSMV-P3)为诱饵,采用酵母双杂交技术(yeast two-hybrid system,Y2HS)从甘蔗酵母文库中筛选到1个编码核酮糖-1,5-二磷酸羧化酶/加氧酶(ribulose-1,5-bisphosphate carboxylase/oxygenase,Rubisco)大亚基的基因,命名为ScRbcL。序列分析表明,该基因开放读码框(open reading frame,ORF)长度为1 337bp,编码478个氨基酸残基。Y2HS和双分子荧光互补(bimolecular flourescence complementation,BiFC)实验表明,ScRbcL与SCSMV-P3存在互作关系。研究认为SCSMV-P3与ScRbcL的互作可能是SCSMV侵染甘蔗导致花叶症状发生的分子基础。Sugarcane streak mosaic virus （SCSMV） is one of the causative agents of sugarcane （Saccharum officinarum L. ） mosaic disease. In this study a sugarcane cDNA library was screened with P3 from SC- SMV as bait using yeast two-hybrid system （Y2HS）. A gene encoding the large subunit of ribulose-1,5-bi- sphosphate carboxylase/oxygenase （Rubisco） was obtained and designed as ScRbcL. The open reading frame （ORF） of ScRbcL is 1 337 bp in length and codes for 478 amino acids. The interaction between the ScRbcL and SCSMV-P3 was identified by Y2HS and bimolecular fluorescence complementation （BiFC）. This research provided new cues to the investigation of the molecular mechanism of SCSMV infection on sugarcane, especially on the mosaic syndrome development caused by sugarcane mosaic disease.

关 键 词：甘蔗条纹花叶病毒 P3蛋白 RUBISCO 互作 

分 类 号：Q789[生物学—分子生物学]