GABA促进成年小鼠SVZ来源的神经干细胞增殖  

GABA Promoted the Proliferation of Neural Stem Cells from the SVZ of Adult Mice

作　　者：晋贞超 邓小兰[1] 王健[2] 郑敏[1] 

机构地区：[1]重庆医科大学干细胞与组织工程研究所,重庆400016 [2]重庆医科大学附属第二医院神经内科,重庆400016

出　　处：《中国细胞生物学学报》2014年第4期489-494,共6页Chinese Journal of Cell Biology

基　　金：国家自然科学基金(批准号:31100985);重庆市科委自然科学基金(批准号:CSTC2010BB5096)资助的课题~~

摘　　要：通过Alamar blue、BrdU掺入免疫荧光双标法、流式细胞术检测了抑制性神经递质GABA(γ-aminobutyric acid)对成年小鼠脑室下区(sub ventricular zone,SVZ)来源的神经干细胞(neural stem cell,NSC)增殖的影响。结果表明,成体NSCs被不同浓度的GABA和BICC干预后,GABA组增殖较空白对照组明显增强,BICC组的增殖则减弱;GABA组作用后增殖活跃期的NSCs比例明显高于空白对照组,BICC组增殖活跃期的NSCs比例明显降低(P<0.05);GABA作用组的处于S-G2期的细胞比例较对照组显著增加(P<0.05)。该研究表明,GABA能促进成年小鼠SVZ区来源的NSCs进入增殖活跃期从而促进其增殖。The Alarmar blue, BrdU incorporation assay and fluorescence activated cell sorter were used to investigate the effect of γ-aminobutyric acid （GABA）, an important inhibitory neurotransmitter in the nervous sys- tem, on proliferation of neural stem ceils （NSCs） from adult mouse subventricular zone （SVZ）. The results showed that with intervention of different concentrations of GABA and BICC, the proliferation of NSCs was significantly enhanced in GABA group, and was decreased in BICC group; In GABA group, the proportion of NSCs in active proliferation period was significantly higher than blank control group, while the proportion in BICC group was sig- nificantly lower （P〈0.05）, and the proportion of cells in S-G2 period was significantly increased （P〈0.05） in GABA group. The results indicated that GABA can promote proliferation of NSCs from SVZ zone of adult mouse by pro- moting them into active proliferation period.

关 键 词：神经干细胞 增殖 Γ-氨基丁酸 

分 类 号：Q42[生物学—神经生物学]