低温胁迫下毛竹叶片色素质量分数与反射光谱的相关性  

Pigment content correlated to reflectance spectrums in Phyllostachys edulis leaves stressed by low temperature

作　　者：许改平[1] 刘芳[1] 吴兴波[1] 温国胜[1] 王玉魁[2] 高岩[1] 高荣孚[1,3] 张汝民[1] 

机构地区：[1]浙江农林大学亚热带森林培育国家重点实验室培育基地,浙江临安311300 [2]国家林业局竹子研究开发中心,浙江杭州310012 [3]北京林业大学生物科学与技术学院,北京100083

出　　处：《浙江农林大学学报》2014年第1期28-36,共9页Journal of Zhejiang A&F University

基　　金：国家自然科学基金资助项目(30972397;31270497;31270756);浙江省科技计划项目(2012F20025);浙江农林大学科研发展基金资助项目(2010FR058)

摘　　要：为了探讨低温处理后毛竹Phyllostachys edulis叶片反射光谱特性与色素质量分数的相互关系,筛选出能够准确监测低温胁迫下毛竹伤害程度的光谱参数。测定了低温处理后毛竹叶片色素质量分数与反射光谱的变化参数,分析叶片光谱反射率、微分光谱及特征参数与色素质量分数的相关性。结果表明:随着温度的降低,叶绿素a和类胡萝卜素质量分数呈下降趋势(P<0.01)。反射光谱参数光谱反射指数、改良红边比、色素比值指数、归一化植被指数、红边归一化指数、改良类胡萝卜素指数和光化学反射指数等均随着温度的降低而降低(P<0.01);红边面积随着胁迫加深不断减小,红边位置向短波方向移动。在绿光区和红光区,叶绿素a和类胡萝卜素质量分数与光谱反射率及微分光谱显著相关(P<0.05),且与大部分光谱参数达到极显著相关(P<0.01),说明反射光谱特征及其参数可用来估算叶片色素质量分数。The reflectance spectrum for photosynthetic pigments of Phyllostachys edulis, a widely distributed, important bamboo with high economic value in China, has a close relationship to the growth process of P. edulis leaves. To evaluate the relationship between reflectance spectra characteristics and pigment content, and to seek sensitive spectral parameters for exact estimations of damage in P. edulis leaves stressed by low temperature, pigment content and reflectance spectra indexes, such as spectral reflectance index wavelength of 705 nm(SR 705), modified spectral ratio( mSR), pigment-specific simple ratio(PSSR), normalized difference vegetation index(NDVI), red NDVI(rNDVI), memory carotenoid reflectance index(Mcri), and photochemical reflectance index(PRI), in P. edulis leaves under low temperature stress were tested and a correlation analysis was used for comparison. Results showed that as temperature deceased, chlorophyll a content and carotenoids in the leaves significantly decreased(P <0.01). As the stress level increased, reflectance spectrum parameters(SR 705, mSR, PSSR, NDVI, rNDVI, Mcri, and PRI)significantly decreased(P<0.01). The red-edge area(S red)decreased, and the red-edge inflection(λ red)position moved toward the short-wave direction with increasing stress. Significant correlations( P <0.05) were found for chlorophyll a content, carotenoids in the leaves and reflectance spectra characteristics, and first derivative of reflectance spectra at the wavelength regions for blue edge and red edge. Moreover, correlations were significant(P<0.01) between pigment content and spectral characteristic parameters of SR 705(r = 0.709), rNDVI(r = 0.724), Mcri(r = 0.706), and S red(r = 0.706). These reflectance spectrum parameters(PSSR, NDVI, rNDVI, and Sred)could be used to evaluate leaf pigment content. [Ch, 4 fig. 5 tab. 40 ref.]

关 键 词：植物学 毛竹 色素 低温胁迫 反射光谱 

分 类 号：S718.43[农业科学—林学] S795.7