玻璃化冻存对鲫鱼卵细胞活性的影响  

Effects of vitrification in crucian carp(Carassius cuvieri) oocytes

作　　者：沈蔷[1] 章海敏[2] 施旆旆 胡军祥[2] 

机构地区：[1]浙江农林大学林业与生物技术学院,浙江临安311300 [2]浙江农林大学动物科技学院,浙江临安311300

出　　处：《浙江农林大学学报》2014年第1期83-88,共6页Journal of Zhejiang A&F University

基　　金：浙江省自然科学基金资助项目(LY13C110001)

摘　　要：以鲫鱼Carassius cuvieri未成熟的卵细胞为材料,研究了玻璃化冻存对鲫鱼卵细胞的存活率及琥珀酸脱氢酶（SDH）活性的影响.结果表明：鲫鱼卵细胞存活率在1～6 d下降明显,6～8 d下降趋势减弱,保持相对稳定;琥珀酸脱氢酶活性呈显著下降（P＜0.05）.玻璃化冻存液VSd组的冻存效果最好,其组分为180.0 g·L-1 1,2-丙二醇,110.0 g·L-1甲醇,0.1 mol· L-1海藻糖和40.0 g·L-1聚乙二醇.该玻璃化冻存液配方及方法在一定时间内保存鲫鱼未成熟卵细胞具有一定的应用价值.The objectives were to analyze the effects of vitrification on the survival rate and succinate dehydrogenase(SDH) activity with immature oocytes in crucian carp(Carassius cuvieri). Results showed that the survival rate decreased significantly within 6 d after vitrification(P>0.05), and SDH activity continued to decrease(P<0.05). Glycol-based vitrification solutions(180.0 g·L-1propylene glycol, 110.0 g·L-1methanol, 0.1 mol·L-1trehalose and 40.0 g·L-1polyethylene glycol) were selected for vitrification due to survival rates and SDH activity. This indicated that with this vitrification solution and method, cryopreservation for immature oocytes of crucian carp was practical. [Ch, 4 fig. 2 tab. 22 ref.]

关 键 词：动物学 卵细胞 玻璃化冻存 玻璃化冻存液 存活率 琥珀酸脱氢酶(SDH) 

分 类 号：S965.117[农业科学—水产养殖] Q959.468[农业科学—水产科学]