不同地理种源无患子的分子多态性分析  

Molecular polymorphic analysis with geographic provenances of Sapindus mukorossi

作　　者：刘宝[1] 范辉华[2] 彭珠清[1] 刘燕[1] 陈存及[1] 

机构地区：[1]福建农林大学林学院,福建福州350002 [2]福建省林业科学研究院,福建福州350012

出　　处：《浙江农林大学学报》2014年第1期151-155,共5页Journal of Zhejiang A&F University

基　　金：国家林业公益性行业科研专项(201104100)

摘　　要：利用筛选出的18对相关序列扩增多态性(SRAP)引物对12个来自印度和中国不同种源的无患子进行SRAP扩增,计算Dice遗传相似系数后用非加权组平均法(UPGMA)法进行聚类,分析其亲缘关系,以便为今后无患子Sapindus mukorossi优质种质资源的保存、选育和后续研究提供参考。试验结果表明:12个样本共扩增出253个带型,其中191条为多态性条带,多态性百分率为75.16%,样本的平均遗传相似系数为0.739,显示出无患子种内的相似度较高,遗传分化不明显。聚类分析表明:印度南的种源与其他种源产生较大的遗传分异,而印度北的种源由于地理纬度与中国国内种源较为相近,因此,和中国国内种源的遗传距离没有印度南种源相差那么大,各种源间的遗传距离与地理距离基本服从正相关的关系。To determine reference material for selective breeding of Sapindus mukorossi, genome DNA of 12 accessions, which were collected from different regions of India and China, were amplified with the sequencerelated amplified polymorphism(SRAP) method using 18 primer pairs. Then, the cumulative band data generated from SRAP was used to calculate the Dice genetic similarity coefficient which was analyzed by the Unweighted Pair Group Method with Arithmetic Mean(UPGMA) to detect their mutual relationships. Genetic diversity was determined with a cluster analysis. Results showed that 191 of the total 253 generated bands were polymorphic(75.16%) with a mean coefficient of 0.739. The cluster analysis showed that the provenance in southern India had greater genetic diversity than other provenances. Compared to the provenance of southern India, less genetic diversity was noted for provenances of northern India and Chinese provenances where geographic latitudes were similar. Positive relationships occurred between genetic distance and geographic distance in 12 geographic provenances of S. mukorossi. Overall, the results showed a low level of diversity in this species. [Ch, 2 fig. 3 tab. 12 ref.]

关 键 词：植物学 无患子 地理种源 相关序列扩增多态性(SRAP) 亲缘关系 分子多态性 

分 类 号：S686[农业科学—观赏园艺] Q943[农业科学—园艺学]