春兰与大花蕙兰杂交后代根状茎增殖与分化条件  

Proliferation and differentiation of rhizomes from a filial generation of Cymbidium goeringii × Cymbidium hybridum

作　　者：孙玉芬[1] 宁惠娟[1] 张韶伊[1] 褚怡[1] 范义荣[1] 

机构地区：[1]浙江农林大学风景园林与建筑学院,浙江临安311300

出　　处：《浙江农林大学学报》2014年第1期156-161,共6页Journal of Zhejiang A&F University

基　　金：浙江省花卉新品种选育重大科技专项重点资助项目(2012C12909-11);浙江省重大科技专项(2009C12087);浙江省重点科技创新团队子项目(2009R50034-10)

摘　　要：为了筛选适合根状茎增殖分化的条件,以春兰Cymbidium goeringii与大花蕙兰Cymbidium hybridum杂交后代的根状茎为材料进行培养,比较了不同基本培养基、植物生长调节物质和有机添加物等对根状茎增殖与分化的影响。结果表明:1/2MS(Murashige and Skoog)培养基对杂交根状茎增殖与分化效果最适宜;2,4-二氯苯氧乙酸(2,4-D)和激动素(KT)对杂交组合春兰与大花蕙兰‘梦境’‘Wanderland’的根状茎分化作用不显著,1/2MS培养基添加1.0mg·L-16-苄基腺嘌呤(6-BA)+1.0 mg·L-1萘乙酸(NAA)对春兰与大花蕙兰‘梦境’的杂交后代增殖分化效果最佳;使用有机添加物对根状茎增殖有显著影响,其中以椰汁增殖效果最好,其次为香蕉泥、土豆汁;添加100 g·L-1香蕉泥+50 mL·L-1椰汁最利于根状茎的增殖与分化。In order to sift out the optimum condition of proliferation and differentiation of rhizomes, using rhizomes from a filial generation of Cymbidium goeringii × C. hybridum, the effects of different basal medium [Murashige and Skoog(MS), 1/2MS, Knudson C(KC), Vacin & Went(VW), B5], plant growth regulators [2,4-D, kinetin(KT), 6-Benzylaminopurine(BA), and 1-Naphthalene acetic acid(NAA)] and organic additives(coconut milk, banana mud, and potato juice) on the proliferation and differentiation of rhizomes were compared. Results showed that 1/2MS was the optimal medium for proliferation of rhizomes. For differentiation of C. goeringii × C. hybridum ‘Wanderland', 2,4-D and KT had little effect; whereas 1/2MS supplemented with 1.0 mg·L-16-BA and 1.0 mg·L-1NAA was the best combination for proliferation and differentiation. The organic additive coconut milk had the strongest influence on proliferation of rhizomes, followed by favorable proliferation and differentiation with 100 g·L-1banana mud and 50 mL·L-1potato juice. [Ch, 1 fig, 4 tab. 16 ref.]

关 键 词：园艺学 兰花 杂交后代 根状茎 增殖 分化 

分 类 号：S682.31[农业科学—观赏园艺]