不同花生品种对卡那霉素的敏感性研究  

Study on Sensitivity of Different Peanut Varieties to Kanamycin

作　　者：张甲佳[1] 张廷婷[2] 徐娟[3] 胡述浩[4] 单世华[2] 郑奕雄[1] 

机构地区：[1]仲恺农业工程学院,广东广州510225 [2]山东省花生研究所,山东青岛266100 [3]湖南农业大学,湖南长沙410000 [4]山东大学(威海),山东威海264200

出　　处：《山东农业科学》2014年第4期36-38,共3页Shandong Agricultural Sciences

基　　金：国家自然科学基金项目(21376281);山东省良种工程项目"花生高产;优质;多抗基因资源发掘与种质创新利用研究";山东省现代农业创新团队花生遗传育种岗位项目

摘　　要：以花生胚小叶为外植体,观察花生胚小叶在添加不同浓度卡那霉素的分化培养基上的生长状况,研究不同花生品种对卡那霉素的敏感性,以确定各品种的卡那霉素临界浓度。结果表明,在供试品种中,D16对卡那霉素最敏感,其临界浓度为100 mg/L;花育22对卡那霉素的耐受性最高,其临界浓度为200 mg/L;J11及鲁花11的卡那霉素临界浓度均为150 mg/L。The cotyledon leaflets of different peanut varieties were used as explants to study their sensi -tivity to kanamycin .Their growth situation in different medium added with different concentrations of kanamy -cin was observed to confirm the critical concentration of kanamycin for each variety .The results were that D16 had the highest sensitivity to Kanamycin , and its critical concentration was 100 mg/L.Huayu 22 had the high-est tolerance to Kanamycin and its critical concentration was 200 mg/L.The critical concentration of J 11 and Luhua 11 to kanamycin were both 150 mg/L.

关 键 词：花生 胚小叶 卡那霉素 敏感性 临界值 

分 类 号：S565.203[农业科学—作物学]