雷公藤内酯醇对AA大鼠脊髓和背根神经节中iNOS,SP表达的影响  

Effect of triptolide on iNOS and SP expressions in spinal dorsal horn and dorsal root ganglion of rats with adjuvant arthritis

作　　者：陈伟[1] 张旭东[1] 逯卓卉[1] 韦登明[1] 

机构地区：[1]宁波大学医学院病理学教研室,浙江宁波315211

出　　处：《中国中药杂志》2014年第9期1675-1679,共5页China Journal of Chinese Materia Medica

基　　金：宁波市药物依赖及共患精神疾病防治创新团队项目(2009B21002);宁波大学王宽诚幸福基金项目

摘　　要：目的:观察不同剂量雷公藤内酯醇(triptolide,TP)对佐剂性关节炎(adjuvant arthritis,AA)大鼠的镇痛效应,及相应节段脊髓背角和背根神经节(dorsal root ganglion,DRG)中诱导型一氧化氮合酶(inducible nitric oxide synthase,iNOS)、P物质(substance P,SP)表达的影响,探讨TP对类风湿性关节炎(RA)镇痛作用的可能机制。方法:选用SD大鼠50只,随机分成正常组(A组)、模型组(B组)、TP低(C组)、中(D组)、高(E组)剂量治疗组。除A组外,每组大鼠右后足跖皮内注入0.1 mL弗氏完全佐剂造模。造模后14 d,C,D,E组大鼠采取不同剂量(C组0.1 mg·kg-1,D组0.2 mg·kg-1,E组0.4 mg·kg-1)TP腹腔注射给药,每天1次,连续给药9 d之后检测50%机械痛阈(mechanical withdraw threshold,MWT),用免疫组织化学方法检测腰5(L5)脊髓背角和DRG节段中iNOS,SP的表达变化情况。结果:B组大鼠50%MWT显著低于A组(P<0.01),经过TP治疗后,C,D,E组热痛阈显著高于B组(P<0.01),TP可显著提高AA大鼠MWT,呈一定的量效关系;免疫组化结果表明,B组iNOS,SP阳性表达水平显著升高(P<0.01),而C,D,E组大鼠L5节段脊髓背角和DRG中iNOS,SP阳性表达水平显著低于B组(P<0.01),呈一定的量效关系。结论:TP对AA具有良好镇痛作用;TP可抑制AA大鼠脊髓背角和DRG中iNOS,SP的表达,这可能是TP产生镇痛作用机制之一。Objective： To observe the analgesic effect of triptolide （TP） of high, middle and low doses on rats with adjuvant arthritis （AA）, and the expressions of inducible nitric oxide synthase （iNOS） and substance P（SP） in spinal dorsal horn and dorsal root ganglion（DRG） of corresponding sections, in order to discuss the possible mechanism for the analgesic effect of TP on rats with adjuvant arthritis. Method： Fifty SD rats were selected and randomly divided into the normal group（ group A）, the model group（ group B）, and TP low（ group C）, middle （group D）, high（ group E） dose groups. Except for the group A, all of the remaining groups were injected with 0. 1 mL of freund＇s complete adjuvant through their right rear toes to establish the model. At 14 d after the model estab- fishment, rats in C, D and E groups were intraperitoneally injected with different doses of TP （0. 1 mg·kg^-1 for the group C, 0.2 mg ·kg^-1for the group D, 0.4 mg ·kg^-1 for the group E） once a day for 9 days. Then the 50% mechanical withdraw threshold （MWT） was determined. And the expressions of iNOS and SP in lumbar5 （L5 ） spinal dorsal horn and DRG were detected with the immunohis- tochemical method. Result： The 50% MWT of rats in the group B was significantly lower than that of the group A（P 〈0. 01 ）. After being treated with TP, the Thermal withdrawal latencies of groups C, D and E were significantly higher than that of the group B（ P 〈 0. 01 ）. TP could notably increase the MWT of AA rats, with a certain dose-effect relationship. The immunohistochemical results indicated that the iNOS and SP expressions significantly increased in the group B （P 〈 0. 01 ）, while the positive expression levels of iNOS and SP in groups C, D and E were significantly lower than that of the group B （ P 〈 0.01 ）, with a certain dose-effect relationship. Conclusion： TP shows a good analgesic effect on AA, and could inhibit the iNOS and SP expressions in spinal dorsal horn and DRG i

关 键 词：佐剂性关节炎 雷公藤内酯醇 镇痛 诱导型一氧化氮合酶 P物质 

分 类 号：R285.5[医药卫生—中药学]