银丹心脑通及主要成分配伍对心肌缺血再灌注损伤的保护作用  

Protection effect of Yindan Xinnaotong capsule and main compositions compatibility on myocardial ischemia /reperfusion injury

作　　者：王万丹[1] 王岚[2] 成龙[3] 殷小杰[2] 许海玉[2] 王建露[2] 梁日欣[2] 杨洪军[2] 

机构地区：[1]首都医科大学中医药学院,北京100069 [2]中国中医科学院中药研究所,北京100700 [3]中国医学科学院药用植物研究所,北京100193

出　　处：《中国中药杂志》2014年第9期1690-1694,共5页China Journal of Chinese Materia Medica

基　　金：国家"重大新药创制"科技重大专项(2012ZX09201201)

摘　　要：目的:研究银丹心脑通及其主要成分配伍对心肌缺血再灌注损伤的保护性作用。方法:采用全心停灌再灌注的方法建立大鼠离体心脏缺血再灌注损伤模型。SD大鼠分为空白组(control),模型组(model),银丹心脑通全方组(YDXNTC),银杏提取物组(GBE),丹参醇提物组(SM-E),丹参水提物组(SM-H),混合物组(灯盏细辛,三七,山楂,绞股蓝,大蒜,天然冰片;MC),银杏+丹参醇提物配伍组,银杏+丹参水提物配伍组,丹参醇提物+丹参水提物配伍组。离体心脏平衡后,空白组正常灌流70 min;模型组正常灌流10 min,停灌30 min,复灌30 min;给药组以含有相应药物的灌流液正常灌流10 min,余同模型组。实验过程中全程记录心电图,观察室性心动过速/室颤(VT/VF)发生率,心脏停跳时间,复跳时间,正常律动时间及心率失常持续时间。复灌结束后,将心肌匀浆,测定SOD活力,MDA,LDH,CK-MB和心肌钙蛋白I(cTnI)释放量。结果:①银丹心脑通全方组,丹参醇提物,丹参水提物,混合物能够延长心脏停跳时间,缩短复跳时间、正常律动时间及心率失常持续时间,但对VT/VF发生的发生没有明显作用。银杏提取物对减少复跳时间、正常律动时间及心率失常持续时间有较好作用,同时能够改善VT/VF的发生。除银杏+丹参水提配伍对心脏停跳时间无明显影响以外,主要成分配伍对心脏停跳时间,复跳时间,正常律动时间及心率失常持续时间均有良好作用,且银杏+丹参醇提对VT/VF发生也有明显作用。②银丹心脑通全方组,银杏提取物,丹参醇提物,银杏+丹参醇提物配伍组,丹参醇提物+丹参水提物配伍组能够提高心肌SOD活力,降低MDA含量;丹参水提物,混合物,银杏+丹参水提物配伍组仅使MDA含量降低。③银丹心脑通全方组和丹参水提物减少了LDH的释放。银杏提取物,丹参醇提物,丹参水提物及银杏+丹参醇提物配伍降低了CK-MB含量。④银杏+丹参醇提物配伍抑�Objective： To study the protected effect of Yindan Xinnaotong capsule （YDXNTC） and main components compati- bility on myocardium ischemia/reperfusion injury. Method： Global ischemia/reperfusion was adopted to induce myocardial ischemia/ reperfusion injury （MIRI） in isolated rat heart. Sprague-Dawley （SD） rats were divided into control, model, YDXNTC, Ginkgo biloba extract （GBE） group, ethanol extract of Salvia miltiorrhiza （SM-E） group, aqueous extract of Salvia miltiorrhiza （SM-H） group, mixed compatibility of other components in YDXNTC （ MC）, GBE and SM-E compatibility （ GSEC）, GBE and SM-H compatibility （ GSHC）, and SM-E and SM-H compatibility （SEHC）. During the experiment, electrocardiogram was recorded to observe cardiac ar- rest time, heart resuscitation time, regaining normal rhythm time, the incidence and duration of arrhythmias （VT/VF）. At the end of reperfusion, hearts were arrested and homogenated to assay the activity of superoxide dismutase （SOD） ,and the content of malondialdehyde （MDA）, lactate dehydrogenase （LDH）, creatine kinase-MB （CK-MB）, cardiac troponin I. Result： ①YDXNTC, SM-E, SM-H and MC elevated cardiac arrest time, also reduced rebeating time, restoring normal rhythm time as well as the duration of ar- rhythmia, but no remarkable impact on VT/VF occurrence. GBE was effective for incidence of VT/VF, also achieved good effect on shortening rebeating time, restoring normal rhythm time and arrhythmia duration. Likewise, obviously reduced rebeating time, restoring normal rhythm time and arrhythmia duration, and evaluated cardiac arrest time were also exhibited in compatibility groups except that no lengthened cardiac arrest time was detected in GSHC. And the incidence of VT/VF was decreased by GSEC. ②YDXNT, ginkgo biloba extract （ GBE）, ethanol extract of salvia miltiorrhiza （ SM-E）, GBE and SM-E compatibility （ GSEC）, and SM-E and aqueous extract of salvia mihiorrhiza （SM-H） compatibility （SEHC） could i

关 键 词：银丹心脑通 主要成分配伍 心肌缺血再灌损伤 保护作用 

分 类 号：R285.5[医药卫生—中药学]