戊己丸活性代表成分在大鼠肝脏中分布的研究  

Reseach on bioactive ingredients in rat liver after oral administration of different combinations of Wuji pill

作　　者：张瑞杰[1] 陈颖[2] 巩仔鹏[2] 董宇[3] 张海先[4] 杨庆[2] 翁小刚[2] 李玉洁[2] 朱晓新[1,2] 

机构地区：[1]首都医科大学中医药学院,北京100069 [2]中国中医科学院中药研究所,北京100700 [3]中国中医科学院广安门医院,北京100053 [4]西南民族大学化学与环境保护工程学院,四川成都610041

出　　处：《中国中药杂志》2014年第9期1695-1703,共9页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(30930114);国家自然科学基金青年基金项目(81001684)

摘　　要：采用L9(34)正交设计将戊己丸中黄连,吴茱萸,白芍提取物组成9组配伍方,以及相应剂量的单味药方,共计18个方。建立并采用UPLC-MS/MS方法测定并比较不同配伍方代表成分小檗碱、巴马汀、吴茱萸碱、吴茱萸次碱、芍药苷在大鼠口服给药2 h后在肝脏中的浓度比较戊己丸不同配伍方中有效成分在大鼠肝脏中的浓度。结果显示戊己丸配伍方中有效成分在肝中的浓度与相同剂量的单味药组方存在明显差异,相同剂量配伍方之间也存在差异。黄连与低、高剂量吴茱萸中吴茱萸碱浓度成正相关,吴茱萸与低剂量黄连中小檗碱的浓度成负相关,与中剂量黄连中小檗碱的浓度成正相关,与中剂量白芍中芍药苷浓度成正相关。白芍与中剂量黄连中巴马汀浓度成负相关、与中剂量吴茱萸中吴茱萸碱、吴茱萸次碱浓度成负相关。提示了黄连、吴茱萸、白芍之间相互作用导致了各活性成分在肝中分布的差异,分析显示各代表成分2 h在肝中浓度最大的配伍比为12∶6∶6。A L9 （34 ） orthogonal design table to be used to get nine combinations of extraction of three herbs of Wuji pill ： Cop- tis chinensis, Tetradium ruticarpum and Paeonia lactiflora Pall. , and nine extraction of single herbs correspondingly, altogether eighteen combinations. Quantification of five representative bioactive ingredients ： berberine, palmatine, evodiamine, rutaecarpine, paeoniflorin in rat liver by ultra high liquid chromatography-tandem mass spectrometry after oral administration at 2 h time point of eighteen combi- nations. The result shows the bioactive ingredients have different concentrations betweem different combinations and the single herb with the same dosage significantly as well as the same dose combinations. C. chinensis with evodiamine concentration of low and high dose T. ruticarpum was positively correlated. T. ruticarpum with berberine concentration of low dose C. chinensis was negatively correlated and of meddle dose C. chinensis was correlated positively. T. ruticarpum with paeoniflorin concentration of middle dose P. lactiflora was correlated positively. P. lactiflora with palmatine concentration of middle dose C. chinensis was negatively correlated and with evo- diamine and rutaecarpine concentration of middle dose T. ruticarpum was negatively correlated. These shows the three single herbs in- teractions resulted in the differences of each ingredients concentration in rat liver. The orthogonal analysis indicates the combination 12： 6：6 make the maximum concentration in rat liver.

关 键 词：戊己丸 配伍 肝脏分布 

分 类 号：R286.0[医药卫生—中药学]