二维斑马鱼模型联合色谱技术高效筛选中药抗骨质疏松活性成分的思路与方法  

Ideas and methods of two-dimensional zebrafish model combined with chromatographic techniques in high-throughput screening of active anti-osteoporosis components of traditional Chinese medicines

作　　者：韦英杰[1,2] 景莉君 詹扬[1] 孙娥[1] 贾晓斌[1] 

机构地区：[1]中国中医科学院江苏分院,江苏省中医药研究院,国家中医药管理局中药口服释药系统重点研究室,江苏南京210028 [2]南京中医药大学附属中西医结合医院,江苏南京210028

出　　处：《中国中药杂志》2014年第9期1739-1742,共4页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(30973978;81274088);江苏省自然科学基金项目(BK2011866);江苏省第十批"六大人才高峰"(2013-YY006)

摘　　要：目的:提出以二维斑马鱼模型联合色谱技术突破评价模型和化合物数量制约,建立高效筛选中药抗骨质疏松活性成分新方法。方法:根据本课题组相关研究及国外有关文献资料,依据斑马鱼骨质疏松模型可高效评价活性,斑马鱼代谢模型可高效富集代谢产物,现代色谱联用技术可高效分离、分析中药成分,提出三者有机联合有望高效解码中药抗骨质疏松体内、外药效物质。结果与结论:该方法使中药成分特别是量微成分与代谢产物的富集、分离分析、在体抗骨质疏松活性筛选简单、高效,充分体现中药药效物质含原形成分及代谢产物,具"多成分,多靶点,整体作用"的特点,为中药抗骨质疏松活性成分的早期、快速发现提供新思路与方法。Objective： To break through the restrictions of the evaluation model and the quantity of compounds by using the two-dimensional zebrafish model combined with chromatographic techniques, and establish a new method for the high-throughpnt screening of active anti-osteoporosis components. Method： According to the research group-related studies and relevant foreign litera- tures, on the basis of the fact that the zebrafish osteoporosis model could efficiently evaluate the activity, the zebrafish metabolism mod- el could efficiently enrich metabolites and the chromatographic techniques could efficiently separate and analyze components of tradition- al Chinese medicines, we proposed that the inherent combination of the three methods is expected to efficiently decode in vivo and in vitro efficacious anti-osteoporosis materials of traditional Chinese medicines. Result and conclusion： The method makes it simple and efficient in the enrichment, separation and analysis on components of traditional Chinese medicines, particularly micro-components and metabolites and the screening anti-osteoporosis activity, fully reflects that efficacious materials of traditional Chinese medicines contain original components and metabolites, with characteristic of ＂multi-components, multi-targets and integral effect＂, which provides new ideas and methods for the early and rapid discovery of active anti-osteoporosis components of traditional Chinese medicines.

关 键 词：斑马鱼 骨质疏松 药物代谢 色谱联用 活性筛选 

分 类 号：R284[医药卫生—中药学]