固相萃取-气相色谱-质谱联用法测定烟草中异丙甲草胺、仲丁灵、氟节胺、敌草胺的残留量  

Determination of Residues of Metolachlor,Butralin,Flumetralin and Napropamide in Tobacco by GC /MS-SPE

作　　者：梅文泉[1] 邵金良[1] 黎其万[1] 陈兴连[1] 

机构地区：[1]云南省农业科学院质量标准与检测技术研究所农业部农产品质量监督检验测试中心(昆明),云南昆明650223

出　　处：《西南农业学报》2014年第2期819-822,共4页Southwest China Journal of Agricultural Sciences

基　　金：中国烟草总公司云南省公司项目"云南绿色烟叶生产研究"(2011YN01)

摘　　要：建立了固相萃取-气相色谱-质谱联用法同时测定烟草中敢草胺、乙丙甲草胺、仲丁灵、氟节胺4种农药残留量的方法.试样经乙腈提取后,采用石墨炭黑-氨基固相萃取小桂净化,然后用GC/MS-SIM法对4种农药进行定量分析.结果表明,采用石墨炭黑-氨基固相萃取小柱净化,能有效去除烟草样品中的色素、油脂等杂质,净化后得到的样液清亮、透明.4种农药在0.025 ～0.5mg/kg添加水平下的回收率为81.8％～108％,相对标准偏差为1.38％～8.95％,乙丙甲草胺、仲丁灵、氟节胺的检出限为0.005mg/kg,敌草胺检出限为0.01 mg/kg.The method for determination of napropamide,metolachlor,butrlin and trifluralin in tobacco was developed.The sample was extracted by acetonitrile,purified with Carb-NH2 solid phase extraction,and quantified the 4 pesticides by GC/MS-SIM.The results showed that it could remove the impurities of pigment,lipin in tobacco using Carb-NH2 solid phase extraction.The sample liquid after purified was clear and translucent.Under these conditions,the average recoveries of 4 pesticides ranged from 81.8 ％ to 108 ％ at spiked level of 0.025 -0.5 mg/kg,and the relative standard deviations were 1.38 ％-8.95 ％.The detection limit of 4 pesticides ranged from 0.005 to 0.01 mg/kg.

关 键 词：烟草 敌草胺 异丙甲草胺 仲丁灵 氟节胺 测定 

分 类 号：S481.8[农业科学—农药学]