低温胁迫后不同回温速度下吉富品系尼罗罗非鱼昏迷率和死亡率研究  

Study of Coma Rate and Mortality of GIFT Tilapia Treated with Different Temperature-increased Speeds after Chilling Stress

作　　者：曾兰 林勇 张永德 杨慧赞 卢其西[2] 陆勤娟[2] 李坤媛[2] 蓝宝同[2] 唐启明[2] 

机构地区：[1]广西壮族自治区水产科学研究院,广西水产遗传育种与健康养殖重点实验室,广西南宁530021 [2]广西师范大学生命科学学院,广西桂林541004

出　　处：《西南农业学报》2014年第2期878-885,共8页Southwest China Journal of Agricultural Sciences

基　　金：自治区直属公益性科研院所基本科研业务费专项资金项目(2060302 GXIF-2011-05,GXIF-2012-5,GXIF-2012-14);国家农业科技成果转化资金项目(2012GB2E100354);广西科学研究与技术开发计划项目(桂科合1298014-7,桂科转122201727);广西区水产畜牧兽医局科研计划项目(09254-01)

摘　　要：应用室内人工降温,以0.2℃/3 h匀速降温至半数致死温度(8.6℃),然后以0.4、0.7和1.0℃/4h升温至室温。对持续时间、累计昏迷率和累计死亡率进行线性回归分析。结果表明:累计昏迷率与持续时间呈显著的线性相关,0.4℃/4 h上升段和下降段试验组线性回归方程分别为:y=-0.982+0.012x和y=0.929-0.005x;0.7℃/4 h上升段和下降段试验组线性回归方程分别为:y=-1.008+0.012x和y=0.789-0.004x;1.0℃/4 h上升段和下降段试验组线性回归方程分别为:y=-0.460+0.006x和y=0.914-0.006x。累计死亡率与持续时间呈显著的线性相关,0.4、0.7和1.0℃/4 h试验组线性回归方程分别为:y=-0.737+0.008x、y=-0.256+0.004x和y=-0.490+0.005x。累计昏迷率与累计死亡率呈显著的线性相关。0.7和1.0℃/4 h组的回温速度可以作为较理想的回温模式,为后续的耐寒选育提供耐寒的罗非鱼亲本。In the condition of indoor artificial cooling,with 0.2 ℃/3 h cooling down to lethal temperature of 50 ％ （8.6℃）,the temperatue rose at the speeds of 0.4,0.7 and 1.0℃/4h to room temperature.The results showed that cumulative coma rate and duration were in significant linear relationship.The linear regression equations between cumulative coma rate and duration in the rising stage and falling stage were：y=-0.982 ＋0.012x and y =0.929-0.005x,y =-1.008 ＋0.012x and y =0.789-0.004x,y =-0.460 ＋0.006x and y =0.914-0.006x,respectively at the temperature-rise speeds of 0.4,0.7 and 1.0℃/4h.The linear regression equations between cumulative mortality and duration were：y =-0.737 ＋ 0.008x,y =-0.256 ＋ 0.004x and y =-0.490 ＋ 0.005x,respectively at the temperature-rise speeds of 0.4 0.7 and 1.0℃/4h.Cumulative coma rate and cumulative mortality were in significant linear relationship.The temperature-rise speeds of 0.7 and 1.0 ℃/4 h can be used as ideal temperature-rise modes,which can provide cold tolerant parents for the subsequent study on cold-resistant breeding of tilapia.

关 键 词：吉富品系尼罗罗非鱼 回温速度 半致死温度 累计昏迷率 累计死亡率 

分 类 号：S965.125[农业科学—水产养殖]