红花新品种‘川红花2号’HPLC指纹图谱研究  

Study on New Variety of Safflower ‘Chuan Honghua 2'HPLC Fingerprint

作　　者：孙佩[1] 童文[1] 杨晓[1] 黄璐琳[1] 胡尚钦[1] 

机构地区：[1]四川省农业科学院经济作物育种栽培研究所,四川简阳641400

出　　处：《西南农业学报》2014年第2期902-904,共3页Southwest China Journal of Agricultural Sciences

基　　金：四川省"十一五"育种攻关项目川产道地药材新品种选育(2006YZGG-12)

摘　　要：为了建立红花新品种‘川红花2号’的HPLC指纹图谱,采用高效液相梯度洗脱方法,对12份‘川红花2号’样品和不同原产地红花材料进行色谱分离,所得图谱经"中药色谱指纹图谱相似度评价系统"处理。结果表明,‘川红花2号’样品间相似度好,均大于0.95,不同原产地红花材料与川红花2号对照指纹图谱相似度低,小于0.93。因此,‘川红花2号’的指纹图谱全面反映了其内在品质,可用于该新品种药材的鉴别和质量控制。只有采用固定的红花品种、固定产地、固定采收期的样品建立的品种专属指纹图谱,才能达到相似度0.95以上。To establish HPLC fingerprint of new variety Safflower‘ Chuan Honghua 2＇,the gradient elution mode was applied to determine ‘ Chuan Honghua 2＇ and different origin safflower samples.Sample＇ s chromatograms were ana]yzed by‘ similarity of TCM chromatographic fingerprint evaluation system＇.The results showed that similarity of ‘ Chuan Honghua 2＇ samples was more than 0.95.Fingerprint similarity between different origin safflower samples and ‘ Chuan Honghua 2＇was below less than 0.93.The HPLC fingerprint of‘ Chuan Honghua 2＇ fully reflected its intrinsic quality and provided identification and controlling the quality of this new variety of safflower.Only the same safflower varieties from the same origin and in the same harvest period could be used to build the exclusive variety fingerprint,the similarity could be more than 0.95.

关 键 词：红花 新品种 高效液相 指纹图谱 

分 类 号：R284.1[医药卫生—中药学]